Key Product Details

Species Reactivity

Feline

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 770626
View all IFN-gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant feline IFN‑ gamma
Gln24-Lys167
Accession # P46402

Specificity

Detects feline IFN‑ gamma in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Feline IFN‑ gamma Antibody

IFN-? antibody in Feline PBMCs by Immunocytochemistry (ICC).

IFN‑ gamma in Feline PBMCs.

IFN-gamma was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Feline IFN-gamma Monoclonal Antibody (Catalog # MAB764) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Feline IFN‑ gamma Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed feline peripheral blood mononuclear cells (PBMCs)

Reviewed Applications

Read 1 review rated 5 using MAB764 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature feline IFN-gamma exists as a noncovalently linked homodimer of 20  25 kDa variably glycosylated subunits (3, 4). It shares 88% amino acid sequence identity with canine IFN-gamma, 72%‑78% with bovine, equine, and porcine IFN‑ gamma, and 40%‑62% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta  subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (5, 6). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (7). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (7, 8). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (9, 10). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (8).

References

  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Argyle, D.J. et al. (1995) DNA Seq. 5:169.
  4. Schijns, V.E. et al. (1995) Immunogenetics 42:440.
  5. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  6. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  7. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  8. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  9. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  10. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Gene Symbol

IFNG

UniProt

P46402

Additional IFN-gamma Products

Product Documents for Feline IFN‑ gamma Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Feline IFN‑ gamma Antibody

For research use only

Customer Reviews for Feline IFN‑ gamma Antibody (1)

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  • Feline IFN-gamma Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Feline
    Verified Customer | Posted 03/04/2022
    Feline peripheral blood mononuclear cells (PBMCs)
    Feline IFN‑ gamma Antibody MAB764

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