Flt-3 Ligand/FLT3L Antibody (4C4) - Azide and BSA Free

Novus Biologicals | Catalog # H00002323-M07

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4C4

Format

Azide and BSA Free
View all Flt-3 Ligand/FLT3L Primary Antibodies »

Product Specifications

Immunogen

FLT3LG (NP_001450, 27 a.a. ~ 136 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TQDCSFQHSPISSDFAVKIRELSDYLLQDYPVTVASNLQDEELCGGLWRLVLAQRWMERLKTVAGSKMQGLLERVNTEIHFVTKCAFQPPPSCLRFVQTNISRLLQETSE

Specificity

FLT3LG - fms-related tyrosine kinase 3 ligand (4C4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Flt-3 Ligand/FLT3L Antibody (4C4) - Azide and BSA Free

ELISA: Flt-3 Ligand/FLT3L Antibody (4C4) [H00002323-M07]

ELISA: Flt-3 Ligand/FLT3L Antibody (4C4) [H00002323-M07]

ELISA: Flt-3 Ligand/FLT3L Antibody (4C4) [H00002323-M07] - Detection limit for recombinant GST tagged FLT3LG is approximately 0.03ng/ml as a capture antibody.
Proximity Ligation Assay: Flt-3 Ligand/FLT3L Antibody (4C4) [H00002323-M07]

Proximity Ligation Assay: Flt-3 Ligand/FLT3L Antibody (4C4) [H00002323-M07]

Proximity Ligation Assay: Flt-3 Ligand/FLT3L Antibody (4C4) [H00002323-M07] - Analysis of protein-protein interactions between KITLG and FLT3LG. HeLa cells were stained with anti-KITLG rabbit purified polyclonal 1:1200 and anti-FLT3LG mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for Flt-3 Ligand/FLT3L Antibody (4C4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Flt-3 Ligand/FLT3L

Flt-3 Ligand, also known as FLT3L, is a cytokine that promotes the differentiation or expansion of multiple hematopoietic cell lineages including monocytes, immature dendritic cells, and early B cell lineage cells. It synergizes with other cytokines to promote the myeloid differentiation and mobilization of hematopoietic stem cells, to induce NK cell development, and to induce terminal B cell maturation. Flt-3 Ligand/FLT3L is expressed as a dimer by T cells and bone marrow and thymic fibroblasts. Both transmembrane and soluble forms of Flt-3 Ligand/FLT3L signal through the tyrosine kinase receptor Flt-3/Flk-2.

Long Name

fms-like Tyrosine Kinase 3 Ligand

Alternate Names

FLG3L, Flt3L, FLT3LG

Entrez Gene IDs

2323 (Human)

Gene Symbol

FLT3LG

UniProt

NP_001450 (Human)

Additional Flt-3 Ligand/FLT3L Products

Product Documents for Flt-3 Ligand/FLT3L Antibody (4C4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Flt-3 Ligand/FLT3L Antibody (4C4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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