FMRP Antibody (2D4) - Azide and BSA Free

Novus Biologicals | Catalog # H00002332-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2D4

Format

Azide and BSA Free
View all FMRP Primary Antibodies »

Product Specifications

Immunogen

FMR1 (NP_002015, 121 a.a. ~ 220 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ATKDTFHKIKLDVPEDLRQMCAKEAAHKDFKKAVGAFSVTYDPENYQLVILSINEVTSKRAHMLIDMHFRSLRTKLSLIMRNEEASKQLESSRQLASRFH

Specificity

FMR1 - fragile X mental retardation 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for FMRP Antibody (2D4) - Azide and BSA Free

Western Blot: FMRP Antibody (2D4) [H00002332-M01]

Western Blot: FMRP Antibody (2D4) [H00002332-M01]

Western Blot: FMRP Antibody (2D4) [H00002332-M01] - Analysis of FMR1 expression in transfected 293T cell line by FMR1 monoclonal antibody (M01), clone 2D4.Lane 1: FMR1 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.
Western Blot: FMRP Antibody (2D4) [H00002332-M01]

Western Blot: FMRP Antibody (2D4) [H00002332-M01]

Western Blot: FMRP Antibody (2D4) [H00002332-M01] - Analysis of FMR1 over-expressed 293 cell line, cotransfected with FMR1 Validated Chimera RNAi ( Cat # H00002332-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FMR1 monoclonal antibody (M01), clone 2D4 (Cat # H00002332-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: FMRP Antibody (2D4) [H00002332-M01]

Western Blot: FMRP Antibody (2D4) [H00002332-M01]

Western Blot: FMRP Antibody (2D4) [H00002332-M01] - FMR1 monoclonal antibody (M01), clone 2D4 Analysis of FMR1 expression in HepG2.
Sandwich ELISA: FMRP Antibody (2D4) [H00002332-M01] - Detection limit for recombinant GST tagged FMR1 is approximately 0.1ng/ml as a capture antibody.

Applications for FMRP Antibody (2D4) - Azide and BSA Free

Application
Recommended Usage

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FMRP

The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome.

Alternate Names

FMRPFRAXAPOF, fragile X mental retardation 1, fragile X mental retardation 1 protein, MGC87458, POF1, premature ovarian failure 1, Protein FMR-1

Entrez Gene IDs

2332 (Human)

Gene Symbol

FMR1

OMIM

309550 (Human)

UniProt

NP_002015 (Human)

Additional FMRP Products

Product Documents for FMRP Antibody (2D4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for FMRP Antibody (2D4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FMRP Antibody (2D4) - Azide and BSA Free

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Protocols

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FAQs

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