Fyn Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32067

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Predicted:

Bovine (99%), Chicken (99%), Porcine (99%), Rat (100%), Xenopus (96%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Fyn Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fyn. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (96%).

Localization

Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Fyn Antibody - BSA Free (NBP1-32067) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Fyn Antibody - BSA Free

Western Blot: Fyn Antibody [NBP1-32067]

Western Blot: Fyn Antibody [NBP1-32067]

Western Blot: Fyn Antibody [NBP1-32067] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Fyn Antibody [NBP1-32067]

Immunocytochemistry/ Immunofluorescence: Fyn Antibody [NBP1-32067]

Immunocytochemistry/Immunofluorescence: Fyn Antibody [NBP1-32067] - Analysis of A431, using FYN antibody at 1:200 dilution.
Fyn Antibody

Western Blot: Fyn Antibody [NBP1-32067] -

Western Blot: Fyn Antibody [NBP1-32067] - Whole zebrafish extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Fyn antibody [N1N3] (NBP1-32067) diluted at 1:1000.
Fyn Antibody

Immunohistochemistry: Fyn Antibody [NBP1-32067] -

Immunohistochemistry: Fyn Antibody [NBP1-32067] - Fyn antibody [N1N3] detects Fyn protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Fyn antibody [N1N3] (NBP1-32067) dilution: 1:200.

Applications for Fyn Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP1-32067 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 40% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Fyn

Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1. The unique N terminal domain of Fyn interacts with the CD3 and eta chains of the TcR. Fyn can bind to other proteins (p82 and p116) through its SH2 and SH3 domains, which may act as substrates or regulators of Fyn activity. Fyn is highly expressed in brain suggesting that it may have a role in the sensory nervous network and in myelination at early stages of CNS formation.

Long Name

FYN oncogene related to SRC, FGR, YES

Alternate Names

SLK, SYN

Gene Symbol

FYN

Additional Fyn Products

Product Documents for Fyn Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Fyn Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for Fyn Antibody - BSA Free (1)

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  • Name: Hongxia Hu
    Application: Western Blot
    Sample Tested: WCL
    Species: Human
    Verified Customer | Posted 09/12/2012
    Fyn Antibody - BSA Free NBP1-32067

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Protocols

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FAQs

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail