GCC185 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-04024

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GCC185 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1 and 50 of human 185 kDa Golgi coiled-coil protein using the numbering given in entry NP_852118.1 (GeneID 9648).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GCC185 Antibody - BSA Free

Western Blot: GCC185 Antibody [NBP2-04024]

Western Blot: GCC185 Antibody [NBP2-04024]

Western Blot: GCC185 Antibody [NBP2-04024] - Detection of human GCC185 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GCC185 antibody NBP2-04024 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. GCC185 was also immunoprecipitated by another rabbit anti-GCC185 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Immunocytochemistry/ Immunofluorescence: GCC185 Antibody [NBP2-04024]

Immunocytochemistry/ Immunofluorescence: GCC185 Antibody [NBP2-04024]

Immunocytochemistry/Immunofluorescence: GCC185 Antibody [NBP2-04024] - PC-3 cells (human) stained with GCC185 antibody (red). Nucleus stained with DAPI (blue). EVOS(TM) M5000 Imaging System - 63x oil objective. ICC/IF image submitted by a verified customer review.

Applications for GCC185 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
This GCC185 antibody is validated for ICC/IF from a verified customer review. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Reviewed Applications

Read 1 review rated 5 using NBP2-04024 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GCC185

GCC185 is encoded by this gene is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Alternate Names

185 kDa Golgi coiled-coil protein, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, GCC protein, 185-kD, GCC185GRIP and coiled-coil domain-containing 2, Golgi coiled-coil protein GCC185, GRIP and coiled-coil domain containing 2, GRIP and coiled-coil domain-containing protein 2, KIAA0336REN53, Ran-binding protein 2-like 4, RANBP2L4, Renal carcinoma antigen NY-REN-53

Gene Symbol

GCC2

UniProt

NP_852118.1 (Human)

Additional GCC185 Products

Product Documents for GCC185 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GCC185 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for GCC185 Antibody - BSA Free (1)

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  • GCC185 Antibody
    Name: Armen Petrosyan
    Application: Immunofluorescence
    Sample Tested: PC-3 human prostate cancer cell line
    Species: Human
    Verified Customer | Posted 08/28/2020
    EVOS™ M5000 Imaging System - 63x oil objective
    PC-3 cells stained with GCC185 Ab - red, blue - nucleus, DAPI.
    GCC185 Antibody - BSA Free NBP2-04024

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