GCH1 Antibody (2C4) - Azide and BSA Free

Novus Biologicals | Catalog # H00002643-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C4

Format

Azide and BSA Free
View all GCH1 Primary Antibodies »

Product Specifications

Immunogen

GCH1 (AAH25415.1, 1 a.a. ~ 250 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEKGPVRAPAEKPRGARCSNGFPERDPPRPGPSRPAEKPPRPEAKSAQPADGWKGERPRSEEDNELNLPNLAAAYSSILSSLGENPQRQGLLKTPWRAASAMQFFTKGYQETISDVLNDAIFDEDHDEMVIVKDIDMFSMCEHHLVPFVGKVHIGYLPNKQVLGLSKLARIVEIYSRRLQVQERLTKQIAVAITEALRPAGVGVVVEATHMCMVMRGVQKMNSKTVTSTMLGVFREDPKTREEFLTLIRS

Specificity

GCH1 - GTP cyclohydrolase 1 (dopa-responsive dystonia)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GCH1 Antibody (2C4) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GCH1 Antibody (2C4) [H00002643-M02]

Immunocytochemistry/ Immunofluorescence: GCH1 Antibody (2C4) [H00002643-M02]

Immunocytochemistry/Immunofluorescence: GCH1 Antibody (2C4) [H00002643-M02] - Analysis of monoclonal antibody to GCH1 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: GCH1 Antibody (2C4) [H00002643-M02] - Detection limit for recombinant GST tagged GCH1 is approximately 0.1ng/ml as a capture antibody.

Applications for GCH1 Antibody (2C4) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control. It has been used for IF.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GCH1

This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described; however, not all variants give rise to a functional enzyme.

Long Name

GTP Cyclohydrolase 1

Alternate Names

DYT5, DYT5a, GTP-CH-1, GTP-CH-I, GTPCH1

Entrez Gene IDs

2643 (Human)

Gene Symbol

GCH1

OMIM

128 (Human)

UniProt

AAH25415 (Human)

Additional GCH1 Products

Product Documents for GCH1 Antibody (2C4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GCH1 Antibody (2C4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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