GIF Antibody (1D9) - Azide and BSA Free

Novus Biologicals | Catalog # H00002694-M03

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D9

Format

Azide and BSA Free
View all GIF Primary Antibodies »

Product Specifications

Immunogen

GIF (NP_005133.2, 318 a.a. ~ 417 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. INNQLRGVELLFNETINVSVKSGSVLLVVLEEAQRKNPMFKFETTMTSWGLVVSSINNIAENVNHKTYWQFLSGVTPLNEGVADYIPFNHEHITANFTQY

Specificity

GIF - gastric intrinsic factor (vitamin B synthesis) (1D9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GIF Antibody (1D9) - Azide and BSA Free

Western Blot: GIF Antibody (1D9) [H00002694-M03]

Western Blot: GIF Antibody (1D9) [H00002694-M03]

Western Blot: GIF Antibody (1D9) [H00002694-M03] - Analysis of GIF transfected lysate using anti-GIF monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GIF rabbit polyclonal antibody.
Immunocytochemistry/ Immunofluorescence: GIF Antibody (1D9) [H00002694-M03]

Immunocytochemistry/ Immunofluorescence: GIF Antibody (1D9) [H00002694-M03]

Immunocytochemistry/Immunofluorescence: GIF Antibody (1D9) [H00002694-M03] - Analysis of monoclonal antibody to GIF on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: GIF Antibody (1D9) [H00002694-M03] - Detection limit for recombinant GST tagged GIF is approximately 0.3ng/ml as a capture antibody.

Applications for GIF Antibody (1D9) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GIF

This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq]

Long Name

Gastric Intrinsic Factor

Alternate Names

IF, IFMH, INF, TCN3

Entrez Gene IDs

2694 (Human)

Gene Symbol

CBLIF

UniProt

NP_005133 (Human)

Additional GIF Products

Product Documents for GIF Antibody (1D9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GIF Antibody (1D9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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