GIT1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-03734

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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all GIT1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 460-640 of human GIT1 (NP_054749.2). EIHKLQAENLQLRQPPGPVPTPPLPSERAEHTPMAPGGSTHRRDRQAFSMYEPGSALKPFGGPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDFHPELESLDGDLDPGLP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Knockout (KO) Validated Rabbit GIT1 Antibody - Azide and BSA Free (NBP3-03734) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for GIT1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GIT1 Antibody - Azide and BSA Free [NBP3-03734]

Immunocytochemistry/ Immunofluorescence: GIT1 Antibody - Azide and BSA Free [NBP3-03734]

Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP3-03734] - Analysis of U-2 OS cells using GIT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

ICC/IF-GIT1 Antibody [NBP3-03734] -

ICC/IF-GIT1 Antibody [NBP3-03734] - Analysis of L929 cells using GIT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
GIT1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GIT1 Antibody - Azide and BSA Free [NBP3-03734] -

Immunocytochemistry/ Immunofluorescence: GIT1 Antibody - Azide and BSA Free [NBP3-03734] - Immunofluorescence analysis of C6 cells using [KO Validated] GIT1 Rabbit pAb (A15437) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western Blot: GIT1 Antibody - Azide and BSA Free [NBP3-03734]

Western Blot: GIT1 Antibody - Azide and BSA Free [NBP3-03734]

Western Blot: GIT1 Antibody [NBP3-03734] - Analysis of extracts of various cell lines, using GIT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Western Blot: GIT1 Antibody - Azide and BSA Free [NBP3-03734]

Western Blot: GIT1 Antibody - Azide and BSA Free [NBP3-03734]

Western Blot: GIT1 Antibody [NBP3-03734] - Analysis of extracts from normal (control) and GIT1 knockout (KO) HeLa cells, using GIT1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Applications for GIT1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:200-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GIT1

G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1.

Long Name

G Protein-Coupled Receptor Kinase Interacting ArfGAP 1

Alternate Names

ARF GAP GIT1

Gene Symbol

GIT1

Additional GIT1 Products

Product Documents for GIT1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GIT1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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