GNAI3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03467

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GNAI3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI3 (NP_006487.1). MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKECGLY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GNAI3 Antibody - BSA Free

Western Blot: GNAI3 AntibodyBSA Free [NBP3-03467]

Western Blot: GNAI3 AntibodyBSA Free [NBP3-03467]

Western Blot: GNAI3 Antibody [NBP3-03467] - Analysis of extracts from normal (control) and GNAI3 knockout (KO) HeLa cells, using GNAI3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western Blot: GNAI3 AntibodyBSA Free [NBP3-03467]

Western Blot: GNAI3 AntibodyBSA Free [NBP3-03467]

Western Blot: GNAI3 Antibody [NBP3-03467] - Analysis of extracts of various cell lines, using GNAI3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Immunoprecipitation: GNAI3 Antibody - BSA Free [NBP3-03467]

Immunoprecipitation: GNAI3 Antibody - BSA Free [NBP3-03467]

Immunoprecipitation: GNAI3 Antibody [NBP3-03467] - Analysis of 200ug extracts of MCF-7 cells, using GNAI3 antibody. Western blot was performed from the immunoprecipitate using this antibody.
GNAI3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GNAI3 Antibody - BSA Free [NBP3-03467] -

Immunocytochemistry/ Immunofluorescence: GNAI3 Antibody - BSA Free [NBP3-03467] - Immunofluorescence analysis of L929 cells using [KO Validated] GNAI3 Rabbit pAb (A13307) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for GNAI3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GNAI3

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in varioustransmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels

Alternate Names

FLJ26559, G(i) alpha-3, guanine nucleotide binding protein (G protein), alpha inhibiting activitypolypeptide 3,87U6, guanine nucleotide-binding protein G(k) subunit alpha

Gene Symbol

GNAI3

Additional GNAI3 Products

Product Documents for GNAI3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GNAI3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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