GRG (Groucho homolog) Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76645

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GRG (Groucho homolog) Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human AES. The immunogen is located within the last 50 amino acids of AES.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRG (Groucho homolog) Antibody - BSA Free

Western Blot: GRG (Groucho homolog) AntibodyBSA Free [NBP1-76645]

Western Blot: GRG (Groucho homolog) AntibodyBSA Free [NBP1-76645]

Western Blot: GRG (Groucho homolog) Antibody [NBP1-76645] - Analysis of GRG (Groucho homolog) in 293 cell lysate with GRG (Groucho homolog) antibody using NBP1-76645 at (A) 1, (B) 2 and (C) 4 ug/ml.
GRG (Groucho homolog) Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GRG (Groucho homolog) Antibody - BSA Free [NBP1-76645] -

Immunocytochemistry/ Immunofluorescence: GRG (Groucho homolog) Antibody - BSA Free [NBP1-76645] - Immunofluorescence of GRG (Groucho homolog) in Hela cells with GRG (Groucho homolog) antibody at 20 ug/mL.
GRG (Groucho homolog) Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GRG (Groucho homolog) Antibody - BSA Free [NBP1-76645] -

Immunocytochemistry/ Immunofluorescence: GRG (Groucho homolog) Antibody - BSA Free [NBP1-76645] - Immunocytochemistry of GRG (Groucho homolog) in HeLa cells with GRG (Groucho homolog) antibody at 10 ug/mL.

Applications for GRG (Groucho homolog) Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Western Blot

1-4 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRG (Groucho homolog)

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

Alternate Names

AES-1, AES-2, Amino enhancer of split, amino-terminal enhancer of split, ESP1, Gp130-associated protein GAM, GRG, GRG5, Protein ESP1, Protein GRG, TLE5

Entrez Gene IDs

166 (Human)

Gene Symbol

TLE5

UniProt

Q08117 (Human)

Additional GRG (Groucho homolog) Products

Product Documents for GRG (Groucho homolog) Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GRG (Groucho homolog) Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GRG (Groucho homolog) Antibody - BSA Free

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Protocols

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