GW182 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03014

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GW182 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 950-1050 of human GW182 (NP_055309.2). QDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAIS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

210 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GW182 Antibody - BSA Free

Western Blot: GW182 AntibodyBSA Free [NBP3-03014]

Western Blot: GW182 AntibodyBSA Free [NBP3-03014]

Western Blot: GW182 Antibody [NBP3-03014] - Analysis of extracts of various cell lines, using GW182 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
Immunocytochemistry/ Immunofluorescence: GW182 Antibody - BSA Free [NBP3-03014]

Immunocytochemistry/ Immunofluorescence: GW182 Antibody - BSA Free [NBP3-03014]

Immunocytochemistry/Immunofluorescence: GW182 Antibody [NBP3-03014] - Analysis of U2OS cells using GW182 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for GW182 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GW182

GW182 (glycine-tryptophan protein of 182 kDa) is a member of the trinucleotide repeat containing 6 protein family. It functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. GW182 has been found to associate with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibition of GW182 expression delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing.

Alternate Names

CAG repeat protein 26, CAGH26FLJ22043, DKFZp666E117, EMSY interactor protein, Glycine-tryptophan protein of 182 kDa, GW182 autoantigen, GW182GW1, KIAA1460EDIE, MGC75384, Protein GW1, TNRC6, trinucleotide repeat containing 6, trinucleotide repeat containing 6A, trinucleotide repeat-containing gene 6A protein

Gene Symbol

TNRC6A

Additional GW182 Products

Product Documents for GW182 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GW182 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GW182 Antibody - BSA Free

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Protocols

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