HIC1 Antibody (2F9) - Azide and BSA Free

Novus Biologicals | Catalog # H00003090-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2F9

Format

Azide and BSA Free
View all HIC1 Primary Antibodies »

Product Specifications

Immunogen

HIC1 (NP_006488, 396 a.a. ~ 453 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YKSSSEETGSSEDPSPPGGHLEGYPCPHLAYGEPESFGDNLYVCIPCGKGFPSSEQLN

Specificity

HIC1 (2F9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HIC1 Antibody (2F9) - Azide and BSA Free

Western Blot: HIC1 Antibody (2F9) [H00003090-M02]

Western Blot: HIC1 Antibody (2F9) [H00003090-M02]

Western Blot: HIC1 Antibody (2F9) [H00003090-M02] - HIC1 monoclonal antibody (M02), clone 2F9 Analysis of HIC1 expression in Jurkat.
Immunocytochemistry/ Immunofluorescence: HIC1 Antibody (2F9) [H00003090-M02]

Immunocytochemistry/ Immunofluorescence: HIC1 Antibody (2F9) [H00003090-M02]

Immunocytochemistry/Immunofluorescence: HIC1 Antibody (2F9) [H00003090-M02] - Analysis of monoclonal antibody to HIC1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: HIC1 Antibody (2F9) [H00003090-M02]

Western Blot: HIC1 Antibody (2F9) [H00003090-M02]

Western Blot: HIC1 Antibody (2F9) [H00003090-M02] - HIC1 monoclonal antibody (M02), clone 2F9. Analysis of HIC1 expression in PC-12.
Sandwich ELISA: HIC1 Antibody (2F9) [H00003090-M02] - Detection limit for recombinant GST tagged HIC1 is approximately 0.1ng/ml as a capture antibody.

Applications for HIC1 Antibody (2F9) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HIC1

Transcriptional repressor. May act as a tumor suppressor. May be involved in development of head, face, limbsand ventral body wall

Alternate Names

Hic-1, hypermethylated in cancer 1, ZBTB29hypermethylated in cancer 1 protein, Zinc finger and BTB domain-containing protein 29, ZNF901

Entrez Gene IDs

3090 (Human)

Gene Symbol

HIC1

OMIM

603825 (Human)

UniProt

NP_006488 (Human)

Additional HIC1 Products

Product Documents for HIC1 Antibody (2F9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HIC1 Antibody (2F9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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