Human 4‑1BB Ligand/TNFSF9 Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # FAB22952AFP680

R&D Systems
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2357A
View all 4-1BB Ligand/TNFSF9 Primary Antibodies »

Product Specifications

Specificity

Detects human 4-1BB Ligand/TNFSF9 in direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Human 4‑1BB Ligand/TNFSF9 Alexa Fluor™ Plus 680‑conjugated Antibody

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: 4-1BB Ligand/TNFSF9

4-1BB Ligand (4-1BBL), also known as CD137L, is a type II transmembrane protein that belongs to the TNF superfamily of molecules and plays an important role in immune response activation (1). The 38 kDa human 4-1BB Ligand consists of an 25 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa extracellular domain (ECD) (2). Within the ECD, human 4-1BB Ligand shares 34% aa sequence identity with mouse and rat 4-1BB Ligand. 4-1BB Ligand is expressed by activated B cells, monocytes, macrophages, dendritic cells (DC), T cells, lymphoma and multiple myeloma cells, hematopoietic stem cells, early myeloid progenitors, neurons, and astrocytes (3-9). A 26 kDa soluble form of 4-1BB Ligand can be released from the surface of activated cells and retains bioactivity (10).
4-1BB Ligand binds to 4-1BB/TNFRSF9/CD137 on activated CD4+ and CD8+ T cells, thymocytes, and NK cells as well as on monocytes, neutrophils, DC, and eosinophils. In response to 4-1BB Ligand binding, 4-1BB transduces a co-stimulatory signal that promotes the proliferation, activation, and survival of CD4+ and CD8+ T cells (4, 11, 12). T cell co-stimulation through CD28 is important for the initial T cell expansion, while 4-1BB acts later in the response (12, 13). 4-1BB Ligand function supports the survival and responsiveness of memory T cells during viral infection (13-15). Reverse signaling through 4-1BB Ligand on monocytes induces the production of inflammatory cytokines (5). On macrophages, 4-1BB Ligand associates in cis with TLR4 and enhances inflammatory cytokine production in response to TLR4 ligation (6). Its expression on early myeloid progenitor cells limits the development of dendritic cells, monocytes, and B cells (9).

References

  1. Wang, C. et al. (2009) Immunol. Rev. 229:192.
  2. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
  3. Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
  4. DeBenedette, M.A. et al. (1997) J. Immunol. 158:551.
  5. Langstein, J. et al. (1998) J. Immunol. 160:2488.
  6. Kang, Y.J. et al. (2007) Nat. Immunol. 8:601.
  7. Baessler, T. et al. (2010) Blood 115:3058.
  8. Gullo, C. et al. (2010) PLoS One 5:e10845.
  9. Lee, S.-W. et al. (2008) Nat. Immunol. 9:917.
  10. Salih, H.R. et al. (2001) J. Immunol. 167:4059.
  11. Wen, T. et al. (2002) J. Immunol. 168:4897.
  12. Cannons, J.L. et al. (2001) J. Immunol. 167:1313.
  13. Bertram, E.M. et al. (2002) J. Immunol. 168:3777.
  14. Bukczynski, J. et al. (2004) Proc. Natl. Acad. Sci. USA 101:1291.
  15. Lin, G.H.Y. et al. (2009) J. Immunol. 182:934.

Alternate Names

41BB Ligand, CD137L, TNFSF9

Entrez Gene IDs

8744 (Human); 21950 (Mouse); 353218 (Rat); 102129676 (Cynomolgus Monkey)

Gene Symbol

TNFSF9

UniProt

P41273.1

Additional 4-1BB Ligand/TNFSF9 Products

Product Documents for Human 4‑1BB Ligand/TNFSF9 Alexa Fluor™ Plus 680‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human 4‑1BB Ligand/TNFSF9 Alexa Fluor™ Plus 680‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Customer Reviews

There are currently no reviews for this product. Be the first to review Human 4‑1BB Ligand/TNFSF9 Alexa Fluor™ Plus 680‑conjugated Antibody and earn rewards!

Have you used Human 4‑1BB Ligand/TNFSF9 Alexa Fluor™ Plus 680‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies