Human AMPK alpha 2 Antibody (847123)

R&D Systems | Catalog # MAB2850

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 847123
View all AMPK alpha 2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human AMPK alpha 2
Phe340-Arg552
Accession # P54646

Specificity

Detects human AMPK alpha 2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human AMPK alpha 1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human AMPK alpha 2 Antibody (847123)

Detection of Human AMPKa2 antibody by Western Blot.

Detection of Human AMPK alpha 2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human AMPKa2 Monoclonal Antibody (Catalog # MAB2850) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for AMPKa2 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human AMPKa2 antibody by Simple WesternTM.

Detection of Human AMPK alpha 2 by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for AMPK alpha 2 at approximately 71 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human AMPK alpha 2 Monoclonal Antibody (Catalog # MAB2850). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
AMPKa2 antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC).

AMPK alpha 2 in HEK293 Human Cell Line.

AMPKa2 was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human AMPKa2 Monoclonal Antibody (Catalog # MAB2850) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human AMPK alpha 2 Antibody (847123)

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line

Simple Western

20 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑468 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AMPK alpha 2

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and delta subunits. The alpha subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinase LKB1. Active AMPK down-regulates anabolic pathways such as fatty acid and cholesterol synthesis, and up-regulates catabolic pathways such as glycolysis and fatty acid oxidation.

Long Name

AMP-activated Protein Kinase alpha

Alternate Names

PRKAA2

Entrez Gene IDs

5563 (Human); 108079 (Mouse); 78975 (Rat)

Gene Symbol

PRKAA2

UniProt

P54646

Additional AMPK alpha 2 Products

Product Documents for Human AMPK alpha 2 Antibody (847123)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human AMPK alpha 2 Antibody (847123)

For research use only

Related Research Areas

Autophagy
Intracellular Kinases
Obesity

Citations for Human AMPK alpha 2 Antibody (847123)

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Protocols

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