Human Angiopoietin-1 DuoSet ELISA

R&D Systems | Catalog # DY923

R&D Systems
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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

156-10000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human Angiopoietin-1 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all Angiopoietin-1 ELISA Kits »

Product Summary for Human Angiopoietin-1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Angiopoietin-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human Angiopoietin-1 DuoSet ELISA

Human Angiopoietin-1 ELISA Standard Curve

Human Angiopoietin-1 ELISA Standard Curve

Kit Contents for Human Angiopoietin-1 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-1

Angiopoietin-1 (Ang-1) is a secreted glycoprotein that plays a critical role in the development and maintenance of the vascular system. It signals through the receptor tyrosine kinase Tie-2 and binds a variety of integrins and the extracellular matrix. Angiopoietin-1 induces the development and branching of blood vessels, promotes vascular endothelial cell motility during angiogenesis, and enhances vascular integrity. It can suppress several VEGF-induced effects on the vasculature. Angiopoietin-1 can protect against pulmonary arterial hypertension, reduce the extent of fibrosis and remodeling in infarcted diabetic myocardium, and enhance tumor progression and metastasis.

Alternate Names

ANGPT1

Entrez Gene IDs

284 (Human); 11600 (Mouse)

Gene Symbol

ANGPT1

Additional Angiopoietin-1 Products

Product Documents for Human Angiopoietin-1 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Angiopoietin-1 DuoSet ELISA

For research use only

Citations for Human Angiopoietin-1 DuoSet ELISA

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  • Human Angiopoietin-1 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Recombinant protein
    Verified Customer | Posted 10/07/2019
    Human Angiopoietin-1 DuoSet ELISA DY923

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