Human Brachyury Antibody [NL557]

R&D Systems | Catalog # NL2085R

R&D Systems
Discontinued Product
NL2085R has been discontinued. View all Brachyury products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, Immunocytochemistry

Label

NL557 (Excitation = 557 nm, Emission = 574 nm)

Antibody Source

Polyclonal Goat IgG
View all Brachyury Primary Antibodies »

Product Specifications

Immunogen

E. coli‑derived, recombinant human Brachyury
Ser2-Glu202
Accession # O15178

Specificity

Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Brachyury Antibody [NL557]

Detection of Brachyury in E9.5 mouse embryo sections.

Brachyury was detected in E9.5 mouse embryo sections using Human Brachyury NL557 conjugated Antibody (Catalog# NL2085R). Embryos were fixed in 4% paraformaldehyde, and blocked with PBS containing 10% normal donkey serum, 0.1% Triton® X 100, and 1% BSA. After blocking, sections were incubated with NL557 conjugated antibody at a final concentration of 1X (1:10 dilution) in blocking buffer for 3 hours at room temperature in the dark. Between each step, cells were washed with PBS containing BSA.

Applications for Human Brachyury Antibody [NL557]

Application
Recommended Usage

Immunocytochemistry

1:10 dilution
Sample: E9.5 mouse embryo sections.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide. See Certificate of Analysis for details.<br> *Contains &lt;0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet&nbsp;(SDS) for additional information and handling instructions.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Brachyury

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Alternate Names

brachyury protein, MGC104817, Protein T, SAVA, T brachyury (mouse) homolog, T brachyury homolog, T Brachyury Transcription Factor, T, brachyury homolog (mouse), T-Box Transcription Factor T, TBXT, TF, TFT

Entrez Gene IDs

6862 (Human)

Gene Symbol

TBXT

UniProt

O15178

Additional Brachyury Products

Product Documents for Human Brachyury Antibody [NL557]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Brachyury Antibody [NL557]

For research use only

Citations for Human Brachyury Antibody [NL557]

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