Human CCL26/Eotaxin-3 Antibody (115002)

R&D Systems | Catalog # MAB653

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 115002
View all CCL26/Eotaxin-3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL26/Eotaxin-3
Thr24-Leu94
Accession # Q9Y258

Specificity

Detects human CCL26/Eotaxin-3 in ELISAs and Western blots. Does not cross-react with recombinant human (rh) CCL11/Eotaxin, recombinant mouse (rm) CCL11/Eotaxin, rhCCL24/Eotaxin-2, rmCCL24/Eotaxin-2, rhCCL3, rhCCL4, rmCCL3, rmCCL4, rhCCL5, rhCCL13, or rhCCL14.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL26/Eotaxin-3 Antibody (115002)

Detection of Human CCL26/Eotaxin-3 antibody by Western Blot.

Detection of Human CCL26/Eotaxin‑3 by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 U/mL Recombinant Human IL-4 (Catalog # 204-IL) for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CCL26/Eotaxin-3 Monoclonal Antibody (Catalog # MAB653) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL26/ Eotaxin-3 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralization by Human CCL26/Eotaxin‑3 Antibody.

Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralization by Human CCL26/Eotaxin‑3 Antibody.

Recombinant Human CCL26/Eotaxin-3 (Catalog # 346-E3) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL26/Eotaxin-3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL26/Eotaxin-3 Monoclonal Antibody (Catalog # MAB653). The ND50 is typically 2-8 µg/mL.
Human CCL26 / Eotaxin-3 ELISA Standard Curve

Human CCL26 / Eotaxin-3 ELISA Standard Curve

Recombinant Human CCL26/Eotaxin‑3 (Catalog # 653-E3) was serially diluted and captured by Mouse Anti-Human CCL26/Eotaxin‑3 Monoclonal Antibody (Catalog # MAB653) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human BMPR‑IA/ALK‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF346) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CCL26/Eotaxin-3 Antibody (115002)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HUVEC human umbilical vein endothelial cells treated with Recombinant Human IL‑4 (Catalog # 204-IL)

Neutralization

Measured by its ability to neutralize CCL26/Eotaxin‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 1 µg/mL Recombinant Human CCL26/Eotaxin‑3.

Human CCL26/Eotaxin-3 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human CCL26/Eotaxin‑3 Biotinylated Antibody (Catalog # BAF346)
  • Standard: Recombinant Human CCL26/Eotaxin-3 Protein (Catalog # 653-E3)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB653 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL26/Eotaxin-3

CCL26, also known as Eotaxin-3, is a CC chemokine that functions as a chemoattractant for eosinophils and activated T cells through interactions with CCR3.

Alternate Names

Eotaxin-3, Eotaxin3, SCYA26

Entrez Gene IDs

10344 (Human)

Gene Symbol

CCL26

UniProt

Q9Y258

Additional CCL26/Eotaxin-3 Products

Product Documents for Human CCL26/Eotaxin-3 Antibody (115002)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL26/Eotaxin-3 Antibody (115002)

For research use only

Citations for Human CCL26/Eotaxin-3 Antibody (115002)

Customer Reviews for Human CCL26/Eotaxin-3 Antibody (115002) (1)

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  • Human CCL26/Eotaxin-3 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 07/19/2019
    Antibody worked well in an EILSA to measure CCL26 in human serum samples.
    Human CCL26/Eotaxin-3 Antibody (115002) MAB653

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