Human CD27 Ligand/TNFSF7 Antibody

R&D Systems | Catalog # AF2738

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CD27 Ligand/TNFSF7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD27 Ligand/TNFSF7
Gln45-Pro193
Accession # AAA36175

Specificity

Detects human CD27 Ligand in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse CD27 Ligand and recombinant human (rh) CD30 Ligand is observed, and less than 1% cross-reactivity with rhCD40 Ligand is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CD27 Ligand/TNFSF7 Antibody

Detection of Human CD27 Ligand/TNFSF7 antibody by Western Blot.

Detection of Human CD27 Ligand/TNFSF7 by Western Blot.

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human CD27 Ligand/TNFSF7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2738) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD27 Ligand/TNFSF7 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of CD27 Ligand/TNFSF7 antibody in Raji Human Cell Line antibody by Flow Cytometry.

Detection of CD27 Ligand/TNFSF7 in Raji Human Cell Line by Flow Cytometry.

Raji human Burkitt's lymphoma cell line was stained with Human CD27 Ligand/TNFSF7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2738, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Detection of CD27Ligand/TNFSF7 antibody in HEK293 Human Cell Line Transfected with Human CD27 Ligand/TNFSF7 antibody by Flow Cytometry.

Detection of CD27Ligand/TNFSF7 in HEK293 Human Cell Line Transfected with Human CD27 Ligand/TNFSF7 by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human CD27 Ligand/TNFSF7 (filled histogram) or irrelevant protein (open histogram) was stained with Mouse Anti-Human CD27 Ligand/TNFSF7 Monoclonal Antibody (Catalog # AF2738) followed by anti-goat PE-conjugated secondary antibody (F0107). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human CD27 Ligand/TNFSF7 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Raji human Burkitt's lymphoma cell line and HEK293 human embryonic kidney cell line transfected with human CD27 Ligand/TNFSF7

Western Blot

1 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line

Reviewed Applications

Read 1 review rated 5 using AF2738 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD27 Ligand/TNFSF7

Human CD27 Ligand (also CD70) is a 30 kDa type 2 transmembrane glycoprotein that is a member of the TNF superfamily. It contains a 20 amino acid (aa) cytoplasmic region and a 155 aa extracellular domain that shows multiple beta -strands. It is an inducible trimer that occurs on B cells, T cells, and NK cells. It regulates T cell dependant B cell differentiation into plasma cells and likely to promotes clonal expansion of T cells. Human CD27 Ligand extracellular region is 64% aa identical to mouse and rat CD27 Ligand extracellular regions.

Alternate Names

CD70, TNFSF7

Entrez Gene IDs

970 (Human); 21948 (Mouse); 301132 (Rat)

Gene Symbol

CD70

UniProt

AAA36175

Additional CD27 Ligand/TNFSF7 Products

Product Documents for Human CD27 Ligand/TNFSF7 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD27 Ligand/TNFSF7 Antibody

For research use only

Customer Reviews for Human CD27 Ligand/TNFSF7 Antibody (1)

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  • Human CD27 Ligand/TNFSF7 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Purified Standard
    Species: Human
    Verified Customer | Posted 11/30/2018

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