Human CD3 GMP Antibody

Catalog #: MAB11411-GMP Datasheet / COA / SDS

Recombinant Monoclonal Antibody. See GMP Antibody Protocols!

Catalog #	Availability	Size / Price	Qty
MAB11411-GMP-100

Save 15% on Select RUO Reagents. See Details.

Recombinant Human CD3 GMP Antibody Stimulates IL-2 Secretion in Jurkat Cells.

1 Image

All CD3 Antibodies

Product Details

Procedure

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD3 GMP Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD3 in a flow cytometry-based assay using Jurkat cells

Source

Recombinant Monoclonal Human IgG₁ Clone # 28497-1

Purification

Protein G purified from cell culture supernatant

Immunogen

Human T lymphocytes

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Aggregation

≤10% aggregation when tested by SEC

Host Cell Protein

≤0.500 ng per μg of antibody when tested by ELISA

Host Cell DNA

≤0.0100 ng per μg of antibody when tested by PCR

Mycoplasma

Negative when tested in a ribosomal RNA hybridization assay

Applications

Recommended Concentration

Sample

T Cell Stimulation

1 µg/mL

Human PBMCs

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

T Cell Stimulation

View Larger

Recombinant Human CD3 GMP Antibody Stimulates IL-2 Secretion in Jurkat Cells. Human CD3 GMP antibody stimulates IL-2 secretion in Jurkat cells (immortalized human T cell line) treated with 5 ng/mL phorbol myristate acetate (PMA). Antibody-mediated stimulation occurred in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA kit. The ED₅₀for this effect is 8.00‑80.0 ng/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CD3

The Bio-Techne CD3 GMP antibody is derived from the OKT3 clone and binds specifically to the epsilon subunit of the CD3 complex. CD3 (cluster of differentiation 3) is a protein complex and T cell co-receptor that is critical for the activation of T cells. Specifically, the CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulin super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum.

Entrez Gene IDs

916 (Human); 12501 (Mouse)

Alternate Names

CD_antigen: CD3e; CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; IMD18; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

Product Datasheets

Product Datasheet

COA

SDS

Manufacturing Specifications

GMP Antibodies

R&D Systems, a Bio-Techne Brand's GMP Antibodies are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products, and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.

R&D Systems' quality focus includes:

Manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
Documented processes and QA control of documentation and process changes
Personnel training programs
Raw material inspection and supplier qualification/monitoring
Fully validated equipment, processes and test methods
Equipment calibration monitored and scheduled using a computerized calibration program
Facility maintenance, safety programs and pest control
Variances dispositioned using material review process
Robust product stability program

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their research. The Certificate of Analysis provided contains the following lot specific information:

Purity (SDS-PAGE)
Aggregation (SEC)
Endotoxin (LAL method, tested per USP<85> and Ph. Eur. 2.6.14 guidelines)
Residual Host Cell Protein and Host Cell DNA
Mycoplasma testing
Post-bottling lot-specific bioassay results (compliance with an established range) and results of microbial testing according to USP <71>

Additional testing and documentation requested by the customer can be arranged at an additional cost.

Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis, Minnesota USA.

R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.