Human CD6 PE-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: CD6

Human CD6 is a 105 kDa (unphosphorylated) -130 kDa (hyperphosphorylated) glycoprotein Group B member of the SRCR (Scavenger Receptor Cys-Rich) superfamily of molecules. It has a restricted expression pattern, being limited to select CD56^dim NK cells, B1a B cells (IgM producers), thymocytes and virtually all resting T cells. Mature CD6 is a type I transmembrane protein 651 amino acids (aa) in length. It contains a 385 aa extracellular region (aa 19-402) that possesses three SRCR repeats, and a relatively long 245 aa cytoplasmic domain. There are at least six alternative splice forms for human CD6, four of which (plus the full-length form) contain an unspliced extracellular region. The isoform used here for immunization (SwissProt: Q8WWJ7) is one of these four, showing splicing only in the cytoplasmic region. Thus, the polyclonal antibody used here will recognize all full-length CD6 molecules, and may, by an absence of staining, suggest the presence of the two isoform variants that are missing the third SRCR repeat that lies between aa 259-361. CD6 has multiple interaction partners. It complexes with CD5 in cis, contributing to the immunological synapse; in trans, it binds to dendritic cell CD166/ALCAM on the surface of adjacent cells. CD6 is also known to bind soluble galectin-1 and -3, and to bind the PAMPs associated with pathogenic microbes. Notably, on T cells and following activation, full-length CD6 is downregulated, and a splice variant lacking the third SRCR is upregulated, abrogating any CD6:CD166 interaction. Functions attributed to CD6 include the induction of chemokine secretion by NK cells, costimulation of antigen recognition by T cells, and an antimicrobial activity attributed to an 85 kDa soluble form of CD6. Over aa 18-398, human and mouse CD6 share 68% aa sequence identity.

Product Datasheets

Related Research Areas

• ITIM/ITAM Immunoreceptors and Related Molecules
• Leukocyte Receptors/Markers in Allergy and Asthma
• Scavenger Receptors
• T Cell Migration/Adhesion