Human CD94 APC-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD94 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human CD94 APC-conjugated Monoclonal Antibody (Catalog # FAB1058A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
CD94 is a 30 kDa type II transmembrane protein with an extracellular C-type lectin domain. It is expressed by natural killer (NK) cells and a subset of CD8+ T cells, with cellular activation resulting in increased cell surface expression. Although CD94 can occur as a non-signaling homodimer, functional activity occurs when CD94 exists as a heterodimer with a NKG2 family member. The CD94/NKG2A heterodimer delivers an inhibitory signal to the expressing cell, whereas, the CD94/NKG2C heterodimer associates with the DAP12 adaptor protein and delivers an activating signal. Both heterodimeric complexes recognize HLA-E with an associated peptide derived from the signal peptide of other HLA proteins.
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