Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1032339
View all CEACAM-20 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived human CEACAM-20
Gln31-Gly450
Accession # Q6UY09-1

Specificity

Detects human CEACAM-20 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CEACAM-20 Antibody

Detection of CEACAM-20 in HEK293 Human Cell Line Transfected with Human CEACAM-20 and eGFP by Flow Cytometry

HEK293 human embryonic kidney cell line transfected with (A) human CEACAM-20 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human CEACAM-20 Monoclonal Antibody (Catalog # MAB9416) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human CEACAM-20 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample:  HEK293 Human Cell Line Transfected with Human CEACAM-20 and eGFP

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CEACAM-20

Carcinoembryonic antigen-related cell adhesion molecule-20 (CEACAM-20) is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. Mature human CEACAM-20 consists of a 420 amino acid (aa) extracellular domain (ECD), a 21 aa helical transmembrane segment, and a 114 aa cytoplasmic domain. The extracellular domain possesses four IgC2-like domains which are stabilized by disulfide bonds, as well as several predicted glycosylation sites (1-5). The extracellular domain of CEACAM-20 is also unique among the CEACAMs because it contains a truncated IgV-like N domain (2). Within the ECD, human CEACAM-20 shares 64% and 62% aa identity with the mouse and rat CEACAM-20, respectively. The cytoplasmic domain is unusually long compared to most other CEACAMs and is predicted to contain four tyrosine phosphorylation sites, two of which correspond to the immune-receptor tyrosine-based activation motif (ITAM) (2, 3). Human CEACAM proteins have been linked to numerous intercellular-adhesion and intracellular signaling processes including cell adhesion, growth, and recognition, differentiation, angiogenesis, and apoptosis (7, 8). Human CEACAM-20 expression is limited to the reproductive system and the intestinal tract, with the highest levels of expression found in the small intestine and prostate (2, 3). An in vitro model of human prostate morphogenesis showed that CEACAM-20 is co-expressed with CEACAM-1 and plays a critical role in the formation of prostate organoids, making it a marker for prostate cancer (2). Although the exact mechanism is not fully understood, CEACAM-20 may promote the proliferation of intestinal epithelial cells (IECs) (9). There is evidence suggesting CEACAM-20 can induce the production of chemokines like interleukin (IL)-8 and stimulate inflammatory responses in colitis and Crohn's disease (6). CEACAM-20 is also thought to act as a physiological substrate for SAP-1 in the intestinal epithelium (10).

References

  1. Tchoupa, A. et al. (2014) J Cell Commun Signal 12:27.
  2. Zhang, H. et al. (2013) PLoS ONE 8:e53359.
  3. Zebhauser, R. et al. (2005) Genomics 86:566.
  4. Beauchemin, N. Arabzadeh, A. (2013) Cancer Metastasis Rev 32(3):643.
  5. Kuespert, K. et al. (2006) Curr Opin Cell Biol 18:565.
  6. Murata, Y. et al. (2015) PNAS E4264.
  7. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
  8. Horst, AK. Wagener, C. (2004) Handb Exp Pharmacol 283.
  9. Kitamura, Y. et al. (2015) Genes to Cells 20:578.
  10. Kotani,  T. et al. (2016) Expert Review of Gastroenterology & Hepatology. 10:1313.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 20

Alternate Names

CEACAM20, GPAD9366, UNQ9366

Entrez Gene IDs

125931 (Human); 71601 (Mouse)

Gene Symbol

CEACAM20

UniProt

Q6UY09-1

Additional CEACAM-20 Products

Product Documents for Human CEACAM-20 Antibody

Certificate of Analysis

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Product Specific Notices for Human CEACAM-20 Antibody

For research use only

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