Human CEBP alpha Antibody (692716)

R&D Systems | Catalog # MAB7094

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 692716
View all CEBP alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CEBP alpha
Met1-Ala124
Accession # P49715

Specificity

Detects human CEBP alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CEBP beta, gamma, delta, epsilon, or zeta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CEBP alpha Antibody (692716)

Detection of Human CEBP a antibody by Western Blot.

Detection of Human CEBP alpha by Western Blot.

Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CEBP a Monoclonal Antibody (Catalog # MAB7094) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CEBP a at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CEBP a antibody in Human Mesenchymal Stem Cells by Immunocytochemistry (ICC).

CEBP alpha in Human Mesenchymal Stem Cells.

CEBP a was detected in immersion fixed human mesenchymal stem cells using Mouse Anti-Human CEBP a Monoclonal Antibody (Catalog # MAB7094) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human CEBP alpha Antibody (692716)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human mesenchymal stem cells

Western Blot

1 µg/mL
Sample: U937 human histiocytic lymphoma cell line

Reviewed Applications

Read 2 reviews rated 5 using MAB7094 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CEBP alpha

CCAAT-enhancer binding protein alpha (CEBP alpha ) is a widely expressed 42 kDa basic-leucine zipper transcription factor that forms functional homodimers and heterodimers with CEBP beta and CEBP gamma. CEBP alpha inhibits mitotic progression through multiple mechanisms and also promotes terminal differentiation of granulocytes and adipocytes. Its activity is regulated by SUMOylation and by phosphorylation at multiple serine residues. CEBP alpha contains three TE transactivation domains (aa 1‑69, aa 70-96, and aa 126-200) and one basic DNA binding motif and leucine zipper (aa 286 - 345). A 30 kDa isoform is generated by the use of an internal translation initiation site. The p30 isoform functions as a dominant negative factor by heterodimerizing with and inhibiting full length CEBP alpha activity. Within aa 1‑124, human CEBP alpha shares 93% amino acid sequence identity with mouse and rat CEBP alpha, respectively.

Long Name

CCAAT/enhancer-Binding Protein alpha

Alternate Names

C/EBP-alpha, CEBPA

Entrez Gene IDs

1050 (Human); 12606 (Mouse); 24252 (Rat)

Gene Symbol

CEBPA

UniProt

P49715 (Human)

Additional CEBP alpha Products

Product Documents for Human CEBP alpha Antibody (692716)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CEBP alpha Antibody (692716)

For research use only

Customer Reviews for Human CEBP alpha Antibody (692716) (2)

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  • Human CEBP  alpha Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Lung fibroblasts
    Species: Human
    Verified Customer | Posted 12/27/2021
    Human CEBP alpha Antibody (692716) MAB7094
  • Human CEBP  alpha Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adipose tissue
    Species: Mouse
    Verified Customer | Posted 10/25/2019
    Human CEBP alpha Antibody (692716) MAB7094

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