Human CHD1 Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB6195S Datasheet / COA / SDS

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FAB6195S-100UG

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Human CHD1 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CHD1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CHD1L, 5, or 7 is observed.

Source

Monoclonal Mouse IgG1 Clone # 677616

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human CHD1
Lys1531-Thr1710
Accession # O14646

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Knockout Validated

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CHD1

CHD1 (Chromohelicase/ATPase DNA-binding protein 1) is a 200-205 kDa member of the SNF2/RAD54 helicase family of proteins. It is an ATP-dependent chromatin remodeling factor that helps maintain chromatin in a transcriptionally active state. In embryonic stem cells, CHD1 associates with the promoters of active genes, a condition that is associated with open chromatin and pluripotency. Human CHD1 is 1710 amino acids (aa) in length. It contains two Ser-rich regions (aa 1-70 and 117‑137), two Chromo (chromatin-organizer-modifer) domains (aa 272-364 and 389-452), a SNF2 family N-terminal domain (aa 484-763) and a C-terminal helicase domain (aa 792-943). There are at least 30 Ser/Thr phosphorylation sites. Over aa 1531-1710, human CHD1 shares 92% aa identity with mouse CHD1.

Long Name

Chromodomain Helicase DNA Binding Protein 1

Entrez Gene IDs

1105 (Human); 12648 (Mouse)

Alternate Names

ATP-dependent helicase CHD1; CHD1; CHD-1; chromodomain helicase DNA binding protein 1; chromodomain-helicase-DNA-binding protein 1; DKFZp686E2337; EC 3.6.1; EC 3.6.4.12