Human CHD1 Antibody (677616)

R&D Systems | Catalog # MAB6195

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 677616
View all CHD1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CHD1
Lys1531-Thr1710
Accession # O14646

Specificity

Detects human CHD1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CHD1L, 5, or 7 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CHD1 Antibody (677616)

Detection of Human and Mouse CHD1 antibody by Western Blot.

Detection of Human and Mouse CHD1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, Raji human Burkitt's lymphoma cell line, and BaF3 mouse pro-B cell line. PVDF Membrane was probed with 0.5 µg/mL of Human CHD1 Monoclonal Antibody (Catalog # MAB6195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CHD1 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CHD1 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

CHD1 in BG01V Human Stem Cells.

CHD1 was detected in immersion fixed BG01V human embryonic stem cells using Human CHD1 Monoclonal Antibody (Catalog # MAB6195) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western Blot Shows Human CHD1 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human CHD1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and CHD1 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CHD1 Monoclonal Antibody (Catalog # MAB6195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CHD1 at approximately 210 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human CHD1 Antibody (677616)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Knockout Validated

CHD1 is specifically detected in HEK293T human embryonic kidney parental parental cell line but is not detectable in CHD1 knockout HEK293T cell line.

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, Raji human Burkitt's lymphoma cell line, and BaF3 mouse pro-B cell line

Reviewed Applications

Read 1 review rated 5 using MAB6195 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CHD1

CHD1 (Chromohelicase/ATPase DNA-binding protein 1) is a 200-205 kDa member of the SNF2/RAD54 helicase family of proteins.  It is an ATP-dependent chromatin remodeling factor that helps maintain chromatin in a transcriptionally active state.  In embryonic stem cells, CHD1 associates with the promoters of active genes, a condition that is associated with open chromatin and pluripotency.  Human CHD1 is 1710 amino acids (aa) in length.  It contains two Ser-rich regions (aa 1-70 and 117‑137), two Chromo (chromatin-organizer-modifer) domains (aa 272-364 and 389-452), a SNF2 family N-terminal domain (aa 484-763) and a C-terminal helicase domain (aa 792-943).  There are at least 30 Ser/Thr phosphorylation sites.  Over aa 1531-1710, human CHD1 shares 92% aa identity with mouse CHD1.    

Long Name

Chromodomain Helicase DNA Binding Protein 1

Alternate Names

ATP-dependent helicase CHD1, CHD-1, chromodomain helicase DNA binding protein 1, chromodomain-helicase-DNA-binding protein 1, DKFZp686E2337, EC 3.6.1, EC 3.6.4.12

Entrez Gene IDs

1105 (Human); 12648 (Mouse)

Gene Symbol

CHD1

UniProt

O14646 (Human)

Additional CHD1 Products

Product Documents for Human CHD1 Antibody (677616)

Certificate of Analysis

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Product Specific Notices for Human CHD1 Antibody (677616)

For research use only

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  • Human CHD1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Osteosarcoma cells
    Species: Human
    Verified Customer | Posted 01/23/2022
    Human CHD1 Antibody (677616) MAB6195

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