Human Claudin-11 Antibody (398205)

R&D Systems | Catalog # MAB4280

R&D Systems
Discontinued Product
MAB4280 has been discontinued. An alternative/replacement product is available: MAB4280R. View all Claudin-11 products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 398205
View all Claudin-11 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Claudin-11
Met1-Val207
Accession # O75508

Specificity

Detects human Claudin‑11 in direct ELISAs and Western blots. Stains human Claudin-11 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Claudin-11 Antibody (398205)

Detection of Claudin‑11 antibody in OVCAR‑3 Human Cell Line antibody by Flow Cytometry.

Detection of Claudin‑11 in OVCAR‑3 Human Cell Line by Flow Cytometry.

OVCAR-3 human ovarian carcinoma cell line was stained with Mouse Anti-Human Claudin-11 Monoclonal Antibody (Catalog # MAB4280, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Applications for Human Claudin-11 Antibody (398205)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: OVCAR‑3 human ovarian carcinoma cell line

Reviewed Applications

Read 1 review rated 4 using MAB4280 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Claudin-11

Claudin-11 is a 207 amino acid multipass membrane protein in the claudin family of epithelial tight junction proteins. Claudin-11 is widely expressed and is involved in epithelial remodeling during spermatogenesis and inner ear mechanotransduction. Claudin-11 is up‑regulated in some cancers. Human Claudin-11 shares 94% aa sequence identity with mouse and rat Claudin-11.

Alternate Names

Claudin11, CLDN11, OSP, OTM

Entrez Gene IDs

5010 (Human); 18417 (Mouse); 84588 (Rat)

Gene Symbol

CLDN11

UniProt

O75508

Additional Claudin-11 Products

Product Documents for Human Claudin-11 Antibody (398205)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Claudin-11 Antibody (398205)

For research use only

Customer Reviews for Human Claudin-11 Antibody (398205) (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human Claudin-11 Antibody (398205)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 的 1 review Showing All
Filter By:
Clear Filters X
  • Human Claudin-11 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: pig SC cells
    Species: Pig
    Verified Customer | Posted 06/07/2017
    Mosue monoclonal anti-clauidn-11 antibody (398205) labeling pig SC cells with Alexa 594
    Human Claudin-11 Antibody (398205) MAB4280

There are no reviews that match your criteria.

Showing  1 - 1 的 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies