COL4A1 (collagen IV alpha 1) is a 185 kDa member of the type IV collagen family. It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes and endothelial cells. Two COL4A1 molecules interact with a 170 kDa alpha 2 chain to form a collagen IV triple helix. This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane. Mature human COL4A1 is 1642 amino acids (aa) in length. It has an N-terminal “7S” proregion (aa 28-172), a central collagenous domain that contains multiple Gly-based repeats (aa 173-1440), and a C-terminal domain that is proteolytically cleaved to generate a 25-28 kDa NC1 globular segment that has potent antiangiogenic activity (aa 1441-1669). Multiple splice forms exist. One shows a deletion of aa 499-849, a second shows a seven aa substitution for aa 513-1669, and a third shows a seven aa substitution for aa 958-1669. Over aa 1441-1669, human COL4A1 is 97% aa identical to mouse COL4A1.
Human Collagen IV alpha 1 Alexa Fluor® 405‑conjugated Antibody
R&D Systems | Catalog # FAB6308V
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ser1441-Thr1669
Accession # P02462
Specificity
Clonality
Host
Isotype
Applications for Human Collagen IV alpha 1 Alexa Fluor® 405‑conjugated Antibody
Immunocytochemistry
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: Collagen IV alpha 1
Alternate Names
Gene Symbol
UniProt
Additional Collagen IV alpha 1 Products
Product Documents for Human Collagen IV alpha 1 Alexa Fluor® 405‑conjugated Antibody
Certificate of Analysis
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Product Specific Notices for Human Collagen IV alpha 1 Alexa Fluor® 405‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars