Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 534625
View all Complement Component C3aR Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human C3a R
Met1-Val482
Accession # Q16581

Specificity

Detects human Complement Component C3a R in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Complement Component C3aR APC‑conjugated Antibody

Detection of Complement Component C3aR in HEK293 human embryonic kidney cell line transfected with human C3aR and eGFP vs irrelevant transfectant by Flow Cytometry

HEK293 human embryonic kidney cell line transfected with human C3aR and eGFP (A) vs irrelevant transfectant (B) were stained with Mouse Anti-Human Complement Component C3aR APC‑conjugated Monoclonal Antibody (Catalog # FAB5995A). View our protocol for Staining Membrane-associated Proteins.

Detection of Complement Component C3a R antibody in Human Whole Blood Monocytes antibody by Flow Cytometry.

Detection of Complement Component C3a R in Human Whole Blood Monocytes by Flow Cytometry.

Human whole blood monocytes were stained with Mouse Anti-Human Complement Component C3a R APC-conjugated Monoclonal Antibody (Catalog # FAB5995A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Complement Component C3aR APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human C3aR and eGFP or Human whole blood monocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Complement Component C3aR

Complement Component 3a receptor (C3a R) is a 70-80 kDa G-protein coupled 7-transmembrane sulfated glycoprotein that belongs to the G-protein coupled receptor 1 family of proteins. It is expressed inducibly or constitutively by platelets, eosinophils, monocytes, astrocytes, CD4+ T cells, dendritic cells, NK cells, mast cells and basophils, neutrophils, adipocytes, osteoclasts, osteoblasts macrophages, vascular endothelial cells, bronchial epithelium and smooth muscle. Engagement of C3a R by  77 kDa C3a has a number of effects that are context-dependent. C3a action on adipocytes facilitates glucose and fatty acid uptake, while in bone, C3a R (plus C5aR) drive the generation of Th1 and Th17 cells. Perhaps its best action is that of a mediator of anaphylaxis. On mast cells it induces histamine release, visceral smooth muscle contraction and vasodilation. Human C3a R extracellular regions share 50% and 42% amino acid identity with mouse and rat C3a R, respectively.

Long Name

Complement Component C3a Receptor

Alternate Names

AZ3B, C3AR, C3AR1, C3R1, Complement Component C3a R

Entrez Gene IDs

719 (Human); 12267 (Mouse)

Gene Symbol

C3AR1

UniProt

Q16581

Additional Complement Component C3aR Products

Product Documents for Human Complement Component C3aR APC‑conjugated Antibody

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Product Specific Notices for Human Complement Component C3aR APC‑conjugated Antibody

For research use only

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