Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Simple Western

Cited:

Neutralization, ELISA Capture, ELISA Development, ELISA Development (Capture), Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 59418
View all CXCL7/NAP-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CXCL7/NAP-2
Ala59-Asp128
Accession # P02775

Specificity

Detects human CXCL7/NAP‑2 in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human (rh) CXCL1, 2, 3, 5, 6, 8, 9, 10, or 12 is observed. In Western blots, no cross-reactivity with rhCXCL1, 2, 3, 5, 6, 8, 9, 10, 12, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12 or recombinant rat CXCL1, 2 or 3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CXCL7/NAP-2 Antibody (59418)

Myeloperoxidase Release Induced by CXCL7/NAP‑2 and Neutralization by Human CXCL7/NAP‑2 Antibody.

Myeloperoxidase Release Induced by CXCL7/NAP‑2 and Neutralization by Human CXCL7/NAP‑2 Antibody.

Recombinant Human CXCL7/NAP-2 (Catalog # 393-NP) induces myeloperox-idase release in the cytochalasin-B treated human neutrophils in a dose-dependent manner (orange line). Myeloperoxidase Release elicited by Recombinant Human CXCL7/NAP-2 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL7/ NAP-2 Monoclonal Antibody (Catalog # MAB393). The ND50 is typically 15-20 µg/mL in the presence of cytochalasin-B.
Detection of Human CXCL7/NAP‑2 antibody by Simple WesternTM.

Detection of Human CXCL7/NAP‑2 by Simple WesternTM.

Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL7/NAP‑2 at approximately 10 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human CXCL7/NAP‑2 Monoclonal Antibody (Catalog # MAB393). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Human CXCL7 / NAP-2 ELISA Standard Curve

Human CXCL7 / NAP-2 ELISA Standard Curve

Recombinant Human CXCL7/NAP‑2 (Catalog # 393-NP) was serially diluted and captured by Mouse Anti-Human CXCL7/NAP‑2 Monoclonal Antibody (Catalog # MAB393) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL7/NAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF393) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CXCL7/NAP-2 Antibody (59418)

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Human platelets

Western Blot

1 µg/mL
Sample: Recombinant Human CXCL7/NAP‑2 (Catalog # 393-NP)

Neutralization

Measured by its ability to neutralize CXCL7/NAP‑2-induced myeloperoxidase release in cytochalasin‑B treated human neutrophils. Schröder, J. M. et al. (1987) J.  Immunol. 139:3474. The Neutralization Dose (ND50) is typically 15-20 µg/mL in the presence of 1 µg/mL Recombinant Human CXCL7/NAP‑2 and cytochalasin‑B.

Human CXCL7/NAP-2 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human CXCL7/NAP‑2 Biotinylated Antibody (Catalog # BAF393)
  • Standard: Recombinant Human CXCL7/NAP-2 Protein (Catalog # 393-NP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB393 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL7/NAP-2

CXCL7, also known as NAP-2, is a 11 kDa angiogenic alpha -chemokine that is produced by the proteolytic cleavage of the inactive 13 kDa platelet basic protein (PBP). PBP, CXCL7, and C-terminally truncated forms of CXCL7 are secreted by monocytes and platelets. Active CXCL7 binds CXCR2 and induces the chemoattration of monocytes, neutrophils, and endothelial progenitor cells. It also inhibits megakaryocytopoiesis in the bone marrow. Within the region included in this immunogen, human CXCL7 shares 52% aa sequence identity with mouse and rat CXCL7.

Alternate Names

CTAP III, NAP-2, NAP2, PPBP

Entrez Gene IDs

5473 (Human)

Gene Symbol

PPBP

UniProt

P02775

Additional CXCL7/NAP-2 Products

Product Documents for Human CXCL7/NAP-2 Antibody (59418)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CXCL7/NAP-2 Antibody (59418)

For research use only

Related Research Areas

Chemokines and Receptors

Citations for Human CXCL7/NAP-2 Antibody (59418)

Customer Reviews for Human CXCL7/NAP-2 Antibody (59418) (1)

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  • Human CXCL7/NAP-2 Antibody
    Name: Michelle Chen
    Application: Block/Neutralize
    Sample Tested: Peripheral blood neutrophils
    Species: Human
    Verified Customer | Posted 04/26/2017

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