Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 986124
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Product Specifications

Immunogen

Human DPCR-1 synthetic peptide
Accession # Q3MIW9

Specificity

Detects human DPCR-1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human DPCR‑1 Antibody

DPCR-1 antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC).

DPCR‑1 in HT‑29 Human Cell Line.

DPCR-1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line (positive control) and not U-87 MG human glioblastoma/astrocytoma cell line (negative control) using Mouse Anti-Human DPCR-1 Monoclonal Antibody (Catalog # MAB33471) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human DPCR‑1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HT‑29 human colon adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DPCR-1

DPCR-1 (Diffuse Panbronchiolitis Critical Region 1) is encoded by a gene that maps to the chromosomal diffuse panbronchiolitis critical region. DPCR-1 is associated with susceptibility to this bronchiolar disease. It has homology with the mucin-like domain of zonadhesin. DPCR-1 is predominantly expressed in lung, kidney, and testis.

Long Name

Diffuse Panbronchiolitis Critical Region 1

Alternate Names

DPCR1, PBLT

Entrez Gene IDs

135656 (Human); 268949 (Mouse); 406199 (Rat)

Gene Symbol

MUCL3

UniProt

Additional DPCR-1 Products

Product Documents for Human DPCR‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human DPCR‑1 Antibody

For research use only

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