DSCAM-L1 is a 250 kDa type I transmembrane Ig superfamily glycoprotein that is predominantly expressed in Purkinje cells and neurons of the cerebral cortex and olfactory bulb.DSCAM-L1 mediates cell adhesion by cation-independent homophilic interactions. It may function in the formation and maintenance of neural networks and play a role in neural disorders such as Gilles de la Tourette and Jacobsen syndromes. Human DSCAM-L1 contains ten Ig-like C2-type domains and six fibronectin type-III domains. An alternately spliced isoform is generated by deletion of 211 amino acids near the N-terminal. Within the extracellular domain, human DSCAM-L1 shares 98% amino acid sequence identity with mouse and rat DSCAM-L1.
Human DSCAM-L1 Antibody
R&D Systems | Catalog # AF3315
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Western Blot, Flow Cytometry, CyTOF-ready
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DSCAM-L1
Glu19-Lys1591
Accession # Q8TD84
Glu19-Lys1591
Accession # Q8TD84
Specificity
Detects human DSCAM-L1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human DSCAM is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human DSCAM-L1 Antibody
Detection of DSCAM‑L1 in A172 Human Cell Line by Flow Cytometry.
A172 human glioblastoma cell line was stained with Goat Anti-Human DSCAM-L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3315, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).Applications for Human DSCAM-L1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: A172 human glioblastoma cell line
Sample: A172 human glioblastoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human DSCAM-L1
Sample: Recombinant Human DSCAM-L1
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: DSCAM-L1
Long Name
Down Syndrome Cell Adhesion Molecule Like 1
Alternate Names
DSCAML1
Gene Symbol
DSCAML1
UniProt
Additional DSCAM-L1 Products
Product Documents for Human DSCAM-L1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human DSCAM-L1 Antibody
For research use only
Related Research Areas
Citations for Human DSCAM-L1 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Western Blot Conditions
- Western Blot Protocol
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- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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