Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Avian, Avian - Chicken, Chicken

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all EGR1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human EGR1
Gln282-Ser433
Accession # P18146

Specificity

Detects human EGR1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human EGR1 Antibody

Detection of EGR1 in SK‑BR‑3 Human Cell Line.

EGR1 was detected in immersion fixed SK‑BR‑3 human breast cancer cell line using Goat Anti-Human EGR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2818) at 5 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the nucleus and the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human EGR1 Antibody

Application
Recommended Usage

Immunocytochemistry

1.7-15 µg/mL
Sample: Immersion fixed SK-BR-3 human breast cancer cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human EGR1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EGR1

Early Growth Response 1, (EGR1) belongs to the EGR family of zinc finger transcription factors and regulates the expression of several tumor suppressors. Its expression is induced by diverse signals that initiate growth and differentiation. EGR1 suppresses or activates cell growth depending on the tumor type. Within the region that includes its three zinc finger domains, human EGR1 shares 99% aa sequence identity with mouse and rat EGR1.

Long Name

Early Growth Response 1

Alternate Names

KROX24, NGFIA, ZNF225

Entrez Gene IDs

1958 (Human)

Gene Symbol

EGR1

UniProt

P18146

Additional EGR1 Products

Product Documents for Human EGR1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human EGR1 Antibody

For research use only

Citations for Human EGR1 Antibody

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Protocols

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