Human Endothelin-3 Antibody (3B6)

R&D Systems | Catalog # MAB5579

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3B6
View all Endothelin-3 Primary Antibodies »

Product Specifications

Immunogen

Human Endothelin-3 synthetic peptide
Accession # P14138

Specificity

Detects human Endothelin‑3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Endothelin-3 Antibody (3B6)

Endothelin-3 antibody in LNCaP Human Cell Line by Immunocytochemistry (ICC).

Endothelin‑3 in LNCaP Human Cell Line.

Endothelin-3 was detected in immersion fixed LNCaP human prostate cancer cell line using Mouse Anti-Human Endothelin-3 Monoclonal Antibody (Catalog # MAB5579) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Endothelin-3 Antibody (3B6)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed LNCaP human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Endothelin-3

Endothelin-1, -2, and -3 are encoded by three separate genes as prepropolypeptides that are cleaved to yield the secreted and inactive big endothelins. Big
Endothelin-3 is further cleaved by Kell blood group antigen endopeptidases to generate the 21 amino acid (aa) bioactive mature peptide. It interacts primarily with the 7TM receptor EDNRB to induce a variety of effects including bronchoconstriction, arteriolar vasoconstriction, gall bladder contraction, insulin and norepinephrine release, and the maintenance and development of melanocytes and neural crest progenitors. Mature human Endothelin-3 shares 100% aa sequence identity with mouse and rat Endothelin-3.

Alternate Names

EDN3, Endothelin3

Entrez Gene IDs

1908 (Human); 13616 (Mouse); 366270 (Rat)

Gene Symbol

EDN3

UniProt

P14138

Additional Endothelin-3 Products

Product Documents for Human Endothelin-3 Antibody (3B6)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Endothelin-3 Antibody (3B6)

For research use only

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