Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunocytochemistry

Cited:

Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 654404
View all ETV5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ETV5
Asn119-Ser223
Accession # P41161

Specificity

Detects human ETV5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ETV1 or rhETV2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human ETV5 Antibody

ETV5 antibody in BT-20 Human Cell Line by Immunocytochemistry (ICC).

ETV5 in BT‑20 Human Cell Line.

ETV5 was detected in immersion fixed BT-20 human breast cancer cell line using Mouse Anti-Human ETV5 Monoclonal Antibody (Catalog # MAB7107) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human ETV5 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BT‑20 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ETV5

ETS translocation variant 5 (ETV5), also known as ERM, is an approximately 70 kDa member of the ETS transcription factor family. The expression of ETV5 in Sertoli, granulosa, and cumulus cells is important for the ability of these cells to support spermatogonia stem cell and oocyte development. ETV5 is upregulated at the invasive front of various carcinomas, and it plays a role in tumor cell invasiveness. It is a target of chromosomal translocation in prostate cancer and forms a fusion protein with TMPRSS2. It is also required for branching morphogenesis during early kidney development. ETV5 contains a single Ets DNA-binding domain (aa 367-452). Within aa 119-223, human ETV5 shares 92% aa sequence identity with mouse and rat ETV5.

Long Name

ETS Translocation Variant 5

Alternate Names

ERM

Entrez Gene IDs

2119 (Human); 104156 (Mouse); 303828 (Rat)

Gene Symbol

ETV5

UniProt

P41161

Additional ETV5 Products

Product Documents for Human ETV5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ETV5 Antibody

For research use only

Related Research Areas

ETS Family Transcription Factors

Citations for Human ETV5 Antibody

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