Human EVI-1 Antibody (702321)

R&D Systems | Catalog # MAB75061

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 702321
View all EVI-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human EVI-1
Gly241-Met430
Accession # Q03112

Specificity

Detects human EVI-1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human EVI-1 Antibody (702321)

EVI-1 antibody in SK-OV-3 Human Cell Line by Immunocytochemistry (ICC).

EVI‑1 in SK‑OV‑3 Human Cell Line.

EVI-1 was detected in immersion fixed SK-OV-3 human ovarian adenocarcinoma cell line using Mouse Anti-Human EVI-1 Monoclonal Antibody (Catalog # MAB75061) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human EVI-1 Antibody (702321)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed SK‑OV‑3 human ovarian adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EVI-1

Ectopic virus integration site 1 (EVI-1), also known as MECOM, is a 145 kDa is a transcriptional regulator that interacts with GATA2 and histone methyltransferases. EVI-1 contains 7 tandem N-terminal zinc finger regions (aa 21-239), a central region, and a cluster of 3 more zinc fingers (aa 733-812). Longer isoforms have 189 aa or 64 aa N-terminal extensions. EVI-1 target genes are critical to hematopoietic stem cell proliferation and myeloid differentiation. EVI-1 is overexpressed in acute myelogenous leukemia (AML) as well as ovarian cancer. Chromosomal translocations fuse EVI-1 with RUNX1 and RPN1 contribute to chromosomal instability, myeloid leukemia proliferation, and a block in myeloid differentiation. Within aa 241-430, human EVI-1 shares 94% aa sequence identity with mouse and rat EVI-1.

Long Name

Ecotropic Viral Integration Site 1

Alternate Names

EVI1, MDS1, MECOM, PRDM3

Entrez Gene IDs

2122 (Human); 14013 (Mouse); 294924 (Rat)

Gene Symbol

MECOM

UniProt

Q03112

Additional EVI-1 Products

Product Documents for Human EVI-1 Antibody (702321)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human EVI-1 Antibody (702321)

For research use only

Related Research Areas

Hematopoietic Stem Cell Markers

Citations for Human EVI-1 Antibody (702321)

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