Human FABP5/E-FABP Antibody

Detection of Human FABP5/E‑FABP by Simple Western^TM.

Simple Western lane view shows lysates of human heart tissue and human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP5/E-FABP at approximately 21 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human FABP5/E‑FABP by Simple Western^TM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FABP5/E-FABP at approximately 21 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human FABP5/E‑FABP Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FABP5/E-FABP knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP5/E-FABP at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse FABP5/E-FABP by Western Blot

Rotenone induced neurotoxicity, especially with co-overexpression of FABP5 and alpha -Synuclein. (A) Representative images of immunoblots probed with antibodies against alpha Syn (~19 kDa) and FABP5 (~12 kDa). Blots with anti-beta -tubulin antibody showed that a similar amount of protein was loaded. (B,C) Viability of Neuro-2A cells treated with indicated concentrations of rotenone for 48 h was assessed using CCK-8 assays. The transfection conditions were pcDNA 3.1-transfected cells (mock) and FABP5-transfected cells in (B); alpha Syn-transfected cells and alpha Syn/FABP5-transfected cells ( alpha S/F5) in (C). Results are presented as means ± SEM (n = 4 parallel cell experiments). * p < 0.05, ** p < 0.01, vs. 0 μM rotenone groups; ## p < 0.01 vs. alpha Syn-transfected cells. CCK-8: cell counting kit-8. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33499263), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse FABP5/E-FABP by Western Blot

Effects of rotenone on alpha Syn oligomerization and aggregation in FABP5/ alpha Syn-transfected Neuro-2A cells. (A–D) Representative images of immunoblots probed with antibodies against alpha Syn and FABP5 under non-denaturing conditions of Triton-soluble and SDS-soluble fractions. Detection with anti-beta -tubulin antibody was used as a protein-loading control. (E–G) Quantitative analyses of protein contents of different molecular weights, which indicate protein complexes of dimer/trimers, oligomers, and aggregates. Results are presented as means ± SEM (n = 3 respective cell experiments). * p < 0.05, ** p < 0.01, vs. 0.1 μM rotenone-treated alpha S/F5 cells. n.s., not statistically significant. D/T: dimers/trimers; O: oligomers; A: aggregates. bk besides blots indicate non-specific bands. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33499263), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse FABP5/E-FABP by Western Blot

Effects of rotenone on alpha Syn oligomerization and aggregation in FABP5/ alpha Syn-transfected Neuro-2A cells. (A–D) Representative images of immunoblots probed with antibodies against alpha Syn and FABP5 under non-denaturing conditions of Triton-soluble and SDS-soluble fractions. Detection with anti-beta -tubulin antibody was used as a protein-loading control. (E–G) Quantitative analyses of protein contents of different molecular weights, which indicate protein complexes of dimer/trimers, oligomers, and aggregates. Results are presented as means ± SEM (n = 3 respective cell experiments). * p < 0.05, ** p < 0.01, vs. 0.1 μM rotenone-treated alpha S/F5 cells. n.s., not statistically significant. D/T: dimers/trimers; O: oligomers; A: aggregates. bk besides blots indicate non-specific bands. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33499263), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse FABP5/E-FABP by Western Blot

Effects of rotenone on alpha Syn oligomerization and aggregation in FABP5/ alpha Syn-transfected Neuro-2A cells. (A–D) Representative images of immunoblots probed with antibodies against alpha Syn and FABP5 under non-denaturing conditions of Triton-soluble and SDS-soluble fractions. Detection with anti-beta -tubulin antibody was used as a protein-loading control. (E–G) Quantitative analyses of protein contents of different molecular weights, which indicate protein complexes of dimer/trimers, oligomers, and aggregates. Results are presented as means ± SEM (n = 3 respective cell experiments). * p < 0.05, ** p < 0.01, vs. 0.1 μM rotenone-treated alpha S/F5 cells. n.s., not statistically significant. D/T: dimers/trimers; O: oligomers; A: aggregates. bk besides blots indicate non-specific bands. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33499263), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse FABP5/E-FABP by Western Blot

Effects of rotenone on alpha Syn oligomerization and aggregation in FABP5/ alpha Syn-transfected Neuro-2A cells. (A–D) Representative images of immunoblots probed with antibodies against alpha Syn and FABP5 under non-denaturing conditions of Triton-soluble and SDS-soluble fractions. Detection with anti-beta -tubulin antibody was used as a protein-loading control. (E–G) Quantitative analyses of protein contents of different molecular weights, which indicate protein complexes of dimer/trimers, oligomers, and aggregates. Results are presented as means ± SEM (n = 3 respective cell experiments). * p < 0.05, ** p < 0.01, vs. 0.1 μM rotenone-treated alpha S/F5 cells. n.s., not statistically significant. D/T: dimers/trimers; O: oligomers; A: aggregates. bk besides blots indicate non-specific bands. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33499263), licensed under a CC-BY license. Not internally tested by R&D Systems.