Human FCRL5 Antibody

R&D Systems | Catalog # AF2087

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FCRL5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FCRL5/FcRH5
Gln16-Arg844
Accession # AAK93971

Specificity

Detects human FCRL5/FcRH5 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) IRTA3, rhIRTA4, and rhIRTA5 is observed and less than 5% cross-reactivity with rhIRTA1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human FCRL5 Antibody

Detection of FCRL5 antibody in CD19+Human PBMC Lymphocytes antibody by Flow Cytometry.

Detection of FCRL5 in CD19+Human PBMC Lymphocytes by Flow Cytometry.

CD19+human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Goat Anti-Human FCRL5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2087, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Applications for Human FCRL5 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: CD19+ human peripheral blood mononuclear cell (PBMC) lymphocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Human FCRL5/FcRH5 (Catalog # 2078-FC)

Reviewed Applications

Read 1 review rated 4 using AF2087 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FCRL5

Fc Receptor-Like 5 (FCRL5), also known as FcRH5, IRTA2, and CD307, is a 120 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL5 binds to purified human IgG with high affinity. Mature human FCRL5 consists of a 836 amino acid (aa) extracellular domain (ECD) with nine Ig-like domains, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain with one immunotyrosine activation motif (ITAM) and two immunotyrosine inhibitory motifs (ITIMs) (1, 3). Mouse FCRL5 contains only five Ig-like domains in its ECD. It shares 49% aa sequence identity with human FCRL5 within common regions. Alternate splicing of human FCRL5 generates isoforms that consist of approximately the first one, six, or eight Ig-like domains (3, 4). FCRL5 expression is restricted to mature B lineage cells in lymphoid tissues and blood (3, 5‑7). Its ligation inhibits signaling through the B cell antigen receptor (8). Epstein-Barr virus transformation of B cells induces the up‑regulation of surface FCRL5 by a direct effect of its EBNA2 protein on FCRL5 gene transcription (9). The FCRL5 gene maps to the 1q21 chromosomal locus, a common site of rearrangements in B cell malignancies, and the FCRL5 protein is preferentially expressed in cell lines with 1q21 abnormalities (3). FCRL5 is up‑regulated on tumor cells in some types of B cell malignancies (6, 10‑12). In addition, soluble FCRL5 is elevated in the serum of many B cell leukemia patients (11, 13).

References

  1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
  2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  3. Hatzivassiliou, G. et al. (2001) Immunity 14:277.
  4. SwissProt # Q96RD9.
  5. Miller, I. et al. (2002) Blood 99:2662.
  6. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
  7. Vidal-Laliena, M. et al. (2005) Cell. Immunol. 236:6.
  8. Haga, C.L. et al. (2007) Proc. Natl. Acad. Sci. 104:9770.
  9. Mohan, J. et al. (2006) Blood 107:4433.
  10. Ise, T. et al. (2005) Clin. Cancer Res. 11:87.
  11. Ise, T. et al. (2007) Leukemia 21:169.
  12. Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.
  13. Ise, T. et al. (2006) Clin. Chem. Lab. Med. 44:594.

Long Name

Fc Receptor-like 5

Alternate Names

BXMAS1, CD307e, FcRH5, IFGP5, IRTA2, PRO820

Entrez Gene IDs

83416 (Human); 329693 (Mouse)

Gene Symbol

FCRL5

UniProt

AAK93971

Additional FCRL5 Products

Product Documents for Human FCRL5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FCRL5 Antibody

For research use only

Customer Reviews for Human FCRL5 Antibody (1)

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  • Human FCRL5/FcRH5 Antibody
    Name: Anonymous
    Application: Immunofluorescence - fresh frozen
    Sample Tested: human tonsil
    Species: Human
    Verified Customer | Posted 11/18/2018
    FcRH5-Alexa647 on fresh-frozen human tonsil
    Human FCRL5 Antibody AF2087

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