Human FCRN Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # FAB8639AFP488

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 937508
View all FCRN Primary Antibodies »

Product Specifications

Specificity

Detects human FCRN in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human FCRN Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

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Spectra Viewer
Spectra Viewer

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FCRN

The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells (1, 2). Mature human FCRN consists of a 274 amino acid (aa) extracellular domain (ECD) with two N-terminal alpha domains, one alpha 3/immunoglobulin-like domain, a 23 aa transmembrane segment, and a 44 aa cytoplasmic domain (3). Within the ECD, human FCRN shares 68% aa sequence identity with mouse and rat FCRN. Mouse FCRN binds with high affinity to IgG from mouse, human, rat, rabbit, guinea pig, bovine, and sheep, while human FCRN binds IgG with significantly lower affinity and is much more restricted in terms of species recognition (4). It does not bind the structurally related chicken IgY (5). FCRN additionally binds to albumin, and both it and IgG are bound at pH 5.0 but not at pH 8.0 (3, 6). FCRN associates noncovalently with beta 2-Microglobulin, and this interaction is important for the intracellular trafficking of FCRN (7-10). FCRN cycles between the plasma membrane and acidified intracellular compartments of endothelial cells and epithelial cells (5, 8). It binds endocytosed IgG and albumin in the low pH vesicles and transports them to the plasma membrane for extracellular release at higher pH. This protects IgG and albumin from lysosomal degradation and helps maintain the circulating levels of both proteins (5, 6). This mechanism is involved in the bidirectional transport of IgG across epithelial and endothelial barriers including neonatal IgG absorption in the intestine and fetal uptake of maternal antibodies through the placenta (5, 8, 11, 12). In the kidney, FCRN recycles albumin to the serum but removes IgG from the glomular basement membrane and promotes its excretion into the urine (13, 14). FCRN is also expressed in neutrophils and myeloid antigen presenting cells (7, 15, 16). It can enhance IgG-mediated phagocytosis and antigen presentation by these cells, but it promotes the degradation of opsonizing IgG rather than returning it to the circulation (15, 16).

References

  1. Baker, K. et al. (2009) Semin. Immunopathol. 31:223.
  2. Baker, K. et al. (2014) Front. Immunol. 5:408.
  3. Story, C.M. et al. (1994) J. Exp. Med. 180:2377.
  4. Ober, R.J. et al. (2001) Int. Immunol. 13:1551.
  5. Dickinson, B.L. et al. (1999) J. Clin. Invest. 104:903.
  6. Chaudhury, C. et al. (2003) J. Exp. Med. 197:315.
  7. Simister, N.E. and K.E. Mostov (1989) Nature 337:184.
  8. Kobayashi, N. et al. (2002) Am. J. Physiol. Renal Physiol. 282:F358.
  9. Praetor, A. and W. Hunziker (2002) J. Cell Sci. 115:2389.
  10. Zhu, X. et al. (2001) J. Immunol. 166:3266.
  11. Spiekermann, G.M. et al. (2002) J. Exp. Med. 196:303.
  12. Firan, M. et al. (2001) Int. Immunol. 13:993.
  13. Sarav, M. et al. (2009) J. Am. Soc. Nephrol. 20:1941.
  14. Akilesh, S. et al. (2008) Proc. Natl. Acad. Sci. 105:967.
  15. Vidarsson, G. et al. (2006) Blood 108:3573.
  16. Qiao, S.-W. et al. (2008) Proc. Natl. Acad. Sci. 105:9337.

Long Name

Neonatal Fc Receptor

Alternate Names

FCGRT

Entrez Gene IDs

2217 (Human); 14132 (Mouse); 29558 (Rat); 102128913 (Cynomolgus Monkey)

Gene Symbol

FCGRT

UniProt

P55899

Additional FCRN Products

Product Documents for Human FCRN Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human FCRN Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Fc Receptors

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