Key Product Details

Species Reactivity

Human

Applications

ELISA Capture (Matched Antibody Pair)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 26019
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Product Specifications

Immunogen

E. coli-derived recombinant human FGF-4
Accession # P08620

Specificity

When used in combination with the biotinylated anti-FGF-4 detection antibody (Catalog # BAF235) in sandwich ELISAs, no significant cross-reactivity or interference was observed with recombinant human (rh) FGF acidic, bFGF acidic, rhFGF basic, bFGF basic, rhFGF-5, rhFGF-6, or rhFGF-7 (KGF).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human FGF-4 Antibody (26019)

Human FGF-4 ELISA Standard Curve

Human FGF-4 ELISA Standard Curve

Recombinant Human FGF‑4 (Catalog # 235-F4) was serially diluted and captured by Mouse Anti-Human FGF‑4 Monoclonal Antibody (Catalog # MAB635) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF235) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human FGF-4 Antibody (26019)

Human FGF-4 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human FGF‑4 Biotinylated Antibody (Catalog # BAF235)
  • Standard: Recombinant Human FGF-4 Protein (Catalog # 235-F4)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-4

FGF-4 is a member of the FGF family of growth factors. FGF-4 is important in embryonic angiogenesis and limb development and is mitogenic for fibroblasts and endothelial cells.

Long Name

Fibroblast Growth Factor 4

Alternate Names

FGF4, HBGF-4, HST-1, KFGF, KS3

Entrez Gene IDs

2249 (Human); 14175 (Mouse); 116499 (Rat)

Gene Symbol

FGF4

UniProt

Additional FGF-4 Products

Product Documents for Human FGF-4 Antibody (26019)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FGF-4 Antibody (26019)

For research use only

Citations for Human FGF-4 Antibody (26019)

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