Human FoxH1 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF4248S Datasheet / COA / SDS

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AF4248S-100UG

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Human FoxH1 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human FoxH1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human FoxB2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FoxH1
Ser164-Ser272
Accession # O75593

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FoxH1

FoxH1 (also known as FAST-1) is a 39 kDa, class 2, subfamily H member of the FOX (forkhead box) family of transcription factors. It is a nuclear protein that binds DNA promoter sites. It does not independently regulate transcription but interacts with SMAD2/4 to activate, and SMAD3/4 to inhibit gene transcription. Human FOXH1 is 365 amino acids (aa) in length. It contains an NLS (aa 22‑30), one FKH (fork head) DNA-binding domain (aa 32‑128) and a SMAD-interaction region (aa 273‑354). Over aa 164‑272, human FOXH1 is 55% identical to mouse FoxH1.

Long Name

Forkhead Box J1

Entrez Gene IDs

8928 (Human)

Alternate Names

FAST1; Fast-1; FAST1forkhead box protein H1; FAST2; Fast-2; Forkhead activin signal transducer 1; Forkhead activin signal transducer 2; forkhead activin signal transducer-1; forkhead box H1; FoxH1; hFAST-1; TGF-beta/activin signal transducer