Human FoxO1/FKHR Antibody

Name: Human FoxO1/FKHR Antibody
Brand: R&D Systems
SKU: AF5939
Price: 159 USD
Availability: InStock

R&D Systems | Catalog # AF5939

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all FoxO1/FKHR Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FoxO1/FKHR
Ala353-Gly655
Accession # Q12778

Specificity

Detects human FoxO1/FKHR in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human FoxO1/FKHR Antibody

Detection of Human FoxO1/FKHR by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human FoxO1/FKHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5939) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxO1/FKHR at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human FoxO1/FKHR by Simple Western^TM.

Simple Western lane view shows lysates of 293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for FoxO1/FKHR at approximately 93 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human FoxO1/FKHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5939) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human FoxO1/FKHR Antibody

Application

Recommended Usage

Simple Western

10 µg/mL
Sample: 293T human embryonic kidney cell line

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line and JEG-3 human epithelial choriocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: FoxO1/FKHR

FoxO1, also called FKHR (forkhead in rhabdomyosarcoma), is a 655 amino acid (aa), 70 kDa, ubiquitously expressed member of the forkhead box O family of winged helix transcription factors. In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferation. In insulin-responsive tissues, nutrient abundance triggers phosphorylation by AKT that blocks nuclear translocation and activity. A 60 kDa form, cleaved at R537, has been found in androgen-treated prostate cancer cells. Over aa 353‑655, human FoxO1/FKHR shares 93% aa identity with mouse and rat FoxO1/FKHR.