Human GFAP Antibody (273807)

R&D Systems | Catalog # MAB2594

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Knockout Validated, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunocytochemistry, Microarray

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 273807
View all GFAP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GFAP
Leu292-Met432
Accession # P14136

Specificity

Detects human GFAP in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human GFAP Antibody (273807)

Detection of Human GFAP antibody by Western Blot.

Detection of Human GFAP by Western Blot.

Western blot shows lysates of human brain (motor cortex) tissue, human brain (cerebellum) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GFAP at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GFAP antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

GFAP in Rat Cortical Stem Cells.

GFAP was detected in immersion fixed differentiated rat cortical stem cells using Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of Human GFAP antibody by Simple WesternTM.

Detection of Human GFAP by Simple WesternTM.

Simple Western lane view shows lysates of human brain (cerebellum) tissue and human brain (motor cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 51-52 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

GFAP Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

GFAP was detected in immersion fixed U937 human histiocytic lymphoma cell line but is not detected in GFAP knockout (KO) U937 Human cell line using Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB2594) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human GFAP Antibody (273807)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed differentiated rat cortical stem cells differentiated for 7 days

Knockout Validated

GFAP was detected in immersion fixed U937 human histiocytic lymphoma cell line but is not detected in GFAP knockout (KO) U937 Human cell line

Simple Western

50 µg/mL
Sample: human brain (cerebellum) tissue and human brain (motor cortex) tissue

Western Blot

1 µg/mL
Sample: Human brain (motor cortex) tissue, human brain (cerebellum) tissue, and human brain (hypothalamus) tissue

Reviewed Applications

Read 3 reviews rated 4.7 using MAB2594 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFAP

Glial Fibrillary Acidic Protein (GFAP) is the predominant component of astrocyte intermediate filaments in the central nervous system. It has also been detected in the glial cells of the enteric nervous system and some Schwann cells in the peripheral nervous systems.

Long Name

Glial Fibrillary Acidic Protein

Alternate Names

ALXDRD, FLJ45472, GFAP, GFAP astrocytes, glial fibrillary acidic protein

Entrez Gene IDs

2670 (Human); 14580 (Mouse); 24387 (Rat)

Gene Symbol

GFAP

UniProt

P14136

Additional GFAP Products

Product Documents for Human GFAP Antibody (273807)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GFAP Antibody (273807)

For research use only

Citations for Human GFAP Antibody (273807)

Customer Reviews for Human GFAP Antibody (273807) (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 的 3 reviews Showing All
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  • Human GFAP Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Astrocytes
    Species: Human
    Verified Customer | Posted 10/10/2021
    Human GFAP Antibody (273807) MAB2594
  • Human GFAP Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Pig TM cells
    Species: Pig
    Verified Customer | Posted 06/07/2017
    Human GFAP antibody labeling Pig TM cells with Alexa488
    Human GFAP Antibody (273807) MAB2594
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 22985741
    Species: Human
    Verified Customer | Posted 02/19/2015

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Protocols

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