Human HNF-6/ONECUT1 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF6277T Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6277T-100UG

Save 15% on Select RUO Reagents. See Details.

Product Details

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HNF-6/ONECUT1 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human HNF-6/ONECUT1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human ONECUT2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HNF-6/ONECUT1
Gln139-Gly283
Accession # Q9UBC0

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: HNF-6/ONECUT1

HNF-6 (also ONECUT1; one "cut" domain family member 1; OC-1) is a monomeric 53-56 kDa member of the One Cut-domain containing class of homeodomain proteins. It is expressed in embryonic hepatic and pancreatic islet anlage, plus adult hepatocytes and intestinal epithelial cells. HNF-6 is a transcriptional activator that is known to interact with Foxa2. Genes regulated by HNF-6 include Glut2, alpha 1AT, alpha -fetoprotein and HNF-3 beta. Human HNF-6 is 465 amino acids (aa) in length. It contains two DNA-binding regions, one classified as a CUT domain (aa 283-369) and another as a homeobox domain (aa 385-444). HNF-6 is both acetylated and phosphorylated, contributing to protein stability and transactivation activity. Over aa 139-283, human HNF 6 shares 99% aa identity with mouse HNF-6. Rat HNF-6 shows a 26 aa insertion after Ala368. Given an overall aa identity of 99% between rat and human, the possibility exists that this insertion may also exist in human.

Long Name

Hepatocyte Nuclear Factor 6

Entrez Gene IDs

3175 (Human); 15379 (Mouse); 25231 (Rat)

Alternate Names

HNF6; HNF-6; HNF-6A; HNF6Ahepatocyte nuclear factor 6, alpha; HNF6hepatocyte nuclear factor 6; HNF-6One cut domain family member 1; OC-1; one cut homeobox 1one cut domain, family member 1; ONECUT1