Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all HOXB1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HOXB1
Met1-Thr192
Accession # P14653

Specificity

Detects human HOXB1 in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) HOXA1, rhHOXB4, and rhHOXA9 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human HOXB1 Antibody

HOXB1 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC).

HOXB1 in NTera‑2 Human Cell Line.

HOXB1 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cells, treated with (upper panel) and without (lower panel) retinoic acid, using Sheep Anti-Human HOXB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6318) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human HOXB1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed NTera‑2 human testicular embryonic carcinoma cells treated with retinoic acid

Reviewed Applications

Read 1 review rated 4 using AF6318 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HOXB1

HOXB1 (Homeobox protein B1; also HOX-2I) is a 32 kDa (predicted) member of the Antp homeobox family, labial subfamily of transcription factors. It is expressed in rhombomere-4 (r-4) of the hindbrain, where it drives the development of r-4 derived structures. Among these are the motor neurons of the facial nerve, and neural crest cells that form Schwann cells plus cartilage and bone of the neck and ear. Human HOXB1 is 301 amino acids (aa) in length. It contains an Antp-type hexapeptide (aa 179-184) that mediates heterodimerization, and a DNA-binding homeobox domain (aa 203-262). There is one potential splice variant that shows a 40 aa substitution for aa 193-301. Over aa 1-192, human HOXB1 shares 82% aa identity with mouse HOXB1.

Long Name

Homeobox B1

Alternate Names

HOX2, HOX2I

Entrez Gene IDs

3211 (Human); 15407 (Mouse); 303491 (Rat)

Gene Symbol

HOXB1

UniProt

P14653 (Human)

Additional HOXB1 Products

Product Documents for Human HOXB1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human HOXB1 Antibody

For research use only

Citations for Human HOXB1 Antibody

Customer Reviews for Human HOXB1 Antibody (1)

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  • Human HOXB1 Antibody
    Name: Lucas Armitage
    Application: Flow Cytometry
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 09/01/2021
    Before use conjugated 100ug of the HOXB1 antibody (AF6318) with Alexa Fluor 488 using the Alexa Fluor™ 488 Antibody Labeling Kit from ThermoFisher and eluted in 100 μL. 293T cells were transfected with a HOXB1 expressing construct, fixed in 4% paraformaldehyde for 10 minutes at room temperature, and permeabilized/stained using BioLegends True-Nuclear™ Transcription Factor Buffer Set according to the instructions. I stained at 1:800 and got robust staining. Data was acquired on a Cytek Aurora and analyzed in FlowJo.
    Human HOXB1 Antibody AF6318

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