Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Adhesion Blockade, Flow Cytometry, CyTOF-ready

Cited:

Direct ELISA, Adhesion Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # Cal 3.10
View all ICAM-3/CD50 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human ICAM-3/CD50 Fc Chimera

Specificity

Detects human ICAM-3/CD50 in Western blots. Does not cross-react with recombinant human (rh) ICAM-1 or rhVCAM-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Human ICAM‑3/CD50 Antibody

Application
Recommended Usage

Adhesion Blockade

Bossy, D. et al. (1995) Eur. J. Immunol. 25:459.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood neutrophils

Western Blot

1 µg/mL
Sample: Recombinant Human ICAM‑3/CD50 Fc Chimera (Catalog # 715-IC)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ICAM-3/CD50

Intercellular Adhesion Molecule-3 (ICAM-3), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha d/ beta 2. ICAM-3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in mice.

References

  1. Hayflick, J. et al. (1998) Immunologic Res. 17:313.

Long Name

Intercellular Adhesion Molecule 3

Alternate Names

CD50, ICAM3

Entrez Gene IDs

3385 (Human)

Gene Symbol

ICAM3

Additional ICAM-3/CD50 Products

Product Documents for Human ICAM‑3/CD50 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ICAM‑3/CD50 Antibody

For research use only

Citations for Human ICAM‑3/CD50 Antibody

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