Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # Cal 3.34
View all ICAM-3/CD50 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human ICAM‑3/CD50 Fc Chimera

Specificity

Detects human ICAM‑3/CD50 in Western blots. ICAM‑3/CD50 was found not to recognize CHO cells expressing the human ICAM-1 protein. ICAM‑3/CD50 was found to recognize CHO cells expressing the human ICAM-3 protein. The epitope for ICAM‑3/CD50 is located on domain 4 of the human ICAM-3 molecule.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human ICAM‑3/CD50 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood monocytes

Western Blot

1 µg/mL
Sample: Recombinant Human ICAM‑3/CD50 Fc Chimera (Catalog # 715-IC)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: ICAM-3/CD50

Intercellular Adhesion Molecule-3 (ICAM-3, CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha d/ beta 2.
ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.

References

  1. Hayflick, J. et al. (1998) Immunologic Res. 17:313.

Long Name

Intercellular Adhesion Molecule 3

Alternate Names

CD50, ICAM3

Entrez Gene IDs

3385 (Human)

Gene Symbol

ICAM3

Additional ICAM-3/CD50 Products

Product Documents for Human ICAM‑3/CD50 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ICAM‑3/CD50 Antibody

For research use only

Customer Reviews for Human ICAM‑3/CD50 Antibody

There are currently no reviews for this product. Be the first to review Human ICAM‑3/CD50 Antibody and earn rewards!

Have you used Human ICAM‑3/CD50 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies