Interleukin-10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair)
Cited:
Immunohistochemistry-Paraffin, Array Development, Bioassay, ELISA Capture, ELISA Development, ELISA Development (Capture)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 127107
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10
Ser19-Asn178
Accession # P22301
Ser19-Asn178
Accession # P22301
Specificity
Detects human IL-10 in ELISAs. In sandwich immunoassays, less than 5% cross-reactivity with recombinant equine IL-10 is observed and less than 0.5% cross-reactivity with recombinant canine IL-10, recombinant mouse IL-10, recombinant rat IL-10, recombinant porcine IL-10, recombinant cotton rat IL-10, and recombinant feline IL-10 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IL-10 Antibody (127107)
Detection of Human IL‑10 by Western Blot.
Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2172) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human IL-10 Antibody (127107)
Application
Recommended Usage
Western Blot
5 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human IL-10
Sample: HEK293 human embryonic kidney cell line transfected with human IL-10
Human IL-10 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 7 reviews rated 4.9 using MAB2172 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-10
Long Name
Interleukin 10
Alternate Names
CSIF, GVHDS, IL10, IL10A, TGIF
Entrez Gene IDs
Gene Symbol
IL10
UniProt
Additional IL-10 Products
Product Documents for Human IL-10 Antibody (127107)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IL-10 Antibody (127107)
For research use only
Related Research Areas
Citations for Human IL-10 Antibody (127107)
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Customer Reviews for Human IL-10 Antibody (127107) (7)
4.9 out of 5
7 Customer Ratings
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Application: ImmunoprecipitationSample Tested: SerumSpecies: HumanVerified Customer | Posted 09/06/2018
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Application: ELISASample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 09/05/2018
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Application: Western BlotSample Tested: Purified proteinSpecies: HumanVerified Customer | Posted 09/05/2018
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Application: Western BlotSample Tested: Purified proteinSpecies: E. coliVerified Customer | Posted 08/10/2018
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Application: ELISASample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 07/17/2018
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Application: Gel ShiftSample Tested: 786-O human renal cell adenocarcinoma cell lineSpecies: HumanVerified Customer | Posted 04/05/2018
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Application: Siteclick antibody biotinylationSample Tested: Monocyte-derived immature dendritic cellsSpecies: MouseVerified Customer | Posted 10/27/2017
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
