Human IL-10 Antibody (21204R)

Name: Human IL-10 Antibody (21204R)
Brand: R&D Systems
SKU: MAB91841
Price: 479 USD
Availability: InStock
Rating: 5 (1 reviews)

R&D Systems | Catalog # MAB91841

(1)

Product Documents

Recombinant Monoclonal Antibody.

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG Clone # 21204R

View all IL-10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-10
Ser19-Asn178
Accession # P22301

Specificity

Detects human IL-10 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-10 or recombinant rat IL-10 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for Human IL-10 Antibody (21204R)

Human IL‑10 ELISA Standard Curve.

Recombinant Human IL-10 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB91841) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2172) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human IL-10 Antibody (21204R)

Application

Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB2172).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IL-10 DuoSet ELISA Kit (Catalog # DY217B) for convenient development of a sandwich ELISA or the Human IL-10 Quantikine ELISA Kit (Catalog # D1000B) for a complete optimized ELISA.

Reviewed Applications

Read 1 review rated 5 using MAB91841 in the following applications:

ELISA (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-10

Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 100034187 (Equine); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)