Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-13 R alpha 1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 1
Ala27-Thr343 (Thr130Ile)
Accession # Q5JSL4

Specificity

Detects human IL‑13 R alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinat human (rh) IL-13 R alpha 2, recombinant mouse IL-13 R alpha 1, rhIL-5 R alpha, rhIL-5 R beta, rhIL-4 R, and rhIL-9 R is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL‑13 R alpha 1 Antibody

Cell Proliferation Induced by IL‑13 and Neutralization by Human IL‑13 R alpha 1 Antibody.

Cell Proliferation Induced by IL‑13 and Neutralization by Human IL‑13 R alpha 1 Antibody.

Recombinant Human IL-13 (213-ILB) stimulates prolif-eration in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-13 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152). The ND50 is typically 1.00-12.0 µg/mL.

Detection of IL‑13 Ra1 antibody in Human Granulocytes antibody by Flow Cytometry.

Detection of IL‑13 R alpha 1 in Human Granulocytes by Flow Cytometry.

Human whole blood granulocytes were stained with Goat Anti-Human IL-13 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152, filled histogram) or control antibody (AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107).

IL-13 Ra1 antibody in Human Skin by Immunohistochemistry (IHC-P).

IL‑13 R alpha 1 in Human Skin.

IL-13 Ra1 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human IL-13 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

IL‑13 R alpha 1 in Human Heart.

IL‑13 R alpha 1 was detected in immersion fixed paraffin-embedded sections of Human Heart using Goat Anti-Human IL‑13 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in myocardial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of IL-13 R alpha 1 by Western Blot

Detection of IL-13 R alpha 1 by Western Blot

IL-4R alpha drives IL-4- and IL-13-mediated signal transduction.N1 immortalized human prostate fibroblasts were treated with vehicle, IL-4 (20ng/ml), or IL-13 (20 ng/m) with or without 2 hr pre-treatment with IL4R alpha (IL4R) (400 ng) or IL-13R alpha 1 (40 ng) antibodies. Both IL-4 (Fig 7A) and IL-13 (Fig 7D) robustly and significantly induced STAT6 phosphoryation compared to vehicle-treated cells. IL-4 stimulation of STAT6 phosphorylation was repressed upon pre-treatment with IL4R alpha (A) but not IL-13R alpha 1 (B) antibodies, whereas IL-13 stimulation of STAT6 phosphorylation was repressed upon pre-treatment with either IL-13R alpha 1 (D) or IL4R alpha (E) antibodies. Densitometric data (S7 Fig) from the replicate experiments is graphed in C (IL-4) and F (IL-13). Significant differences are indicated as * p <.05; ** p <.01; *** p <.001; **** p <.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36201508), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-13 R alpha 1 by Western Blot

Detection of IL-13 R alpha 1 by Western Blot

IL-4R alpha drives IL-4- and IL-13-mediated signal transduction.N1 immortalized human prostate fibroblasts were treated with vehicle, IL-4 (20ng/ml), or IL-13 (20 ng/m) with or without 2 hr pre-treatment with IL4R alpha (IL4R) (400 ng) or IL-13R alpha 1 (40 ng) antibodies. Both IL-4 (Fig 7A) and IL-13 (Fig 7D) robustly and significantly induced STAT6 phosphoryation compared to vehicle-treated cells. IL-4 stimulation of STAT6 phosphorylation was repressed upon pre-treatment with IL4R alpha (A) but not IL-13R alpha 1 (B) antibodies, whereas IL-13 stimulation of STAT6 phosphorylation was repressed upon pre-treatment with either IL-13R alpha 1 (D) or IL4R alpha (E) antibodies. Densitometric data (S7 Fig) from the replicate experiments is graphed in C (IL-4) and F (IL-13). Significant differences are indicated as * p <.05; ** p <.01; *** p <.001; **** p <.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36201508), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human IL‑13 R alpha 1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human whole blood granulocytes

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin and human heart.

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑13 R alpha 1 Fc Chimera (Catalog # 146-IR)

Neutralization

Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 1.00-12.0 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑13.

Reviewed Applications

Read 2 reviews rated 3 using AF152 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-13 R alpha 1

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2. The human IL-13 R alpha 1 was originally cloned based on sequence homology to the mouse IL-13 R alpha 1. The IL‑13 R alpha 1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region and a 59 aa residue cytoplasmic tail. Human and mouse IL-13 R alpha 1 share 76% aa sequence identity. The extracellular domain of IL‑13 R alpha 1 is also closely related to that of IL-13 R alpha 2. IL-13 R alpha 1 has been shown to combine with the IL-4 R alpha to form a high-affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated.

References

  1. Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
  2. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
  3. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
  4. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.
  5. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.

Long Name

Interleukin 13 Receptor alpha 1

Alternate Names

CD213a1, IL-13Ra1, IL13R alpha 1, IL13RA1

Entrez Gene IDs

3597 (Human); 16164 (Mouse)

Gene Symbol

IL13RA1

UniProt

Q5JSL4

Additional IL-13 R alpha 1 Products

Product Documents for Human IL‑13 R alpha 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL‑13 R alpha 1 Antibody

For research use only

Citations for Human IL‑13 R alpha 1 Antibody

Customer Reviews for Human IL‑13 R alpha 1 Antibody (2)

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  • Human IL-13 R alpha 1 Antibody
    Name: Kurt Shanebeck
    Application: Block/Neutralize
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 12/06/2017
    Bio-Techne Response
    Technical Service will follow up with this customer
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 22092872
    Species: Human
    Verified Customer | Posted 01/05/2015

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