Human IL-16 Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
IC3161N-100UG
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Human IL-16 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-16.
Source
Monoclonal Mouse IgG2b Clone # 70720
Immunogen
E. coli-derived recombinant human IL-16 isoform 1
Met1203-Ser1332
Accession # Q14005
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Raji human Burkitt's lymphoma cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-16

Interleukin 16, also named lymphocyte chemoattractant factor (LCF), was originally identified as a CD8+ T-cell-derived chemoattractant for CD4+ cells. The biologically active form of IL-16 was originally proposed to be a homotetramer of 14 kDa chains containing 130 amino acid residue subunits. The complete pro-IL-16 cDNA was subsequently cloned and shown to encode a 631 amino acid residue hydrophilic protein that lacked a signal peptide. The original 130 amino acid residue polypeptide is now believed to have been derived from the C terminus of the precursor. IL-16 precursor protein has been detected in the lysates of various cells including mitogen stimulated PBMCs. The biologically active and secreted natural IL-16 is assumed to be a proteolytic cleavage product of pro-IL-16 generated by proteases present in or on activated CD8+ cells. A likely cleavage site was proposed to be at aspartate residue 510. This would yield a 121 amino acid residue protein, smaller than the 130 aa residue protein first described. The expression of IL-16 precursor mRNA has been detected in various tissues including spleen, thymus, lymph nodes, peripheral leukocytes, bone marrow and cerebellum. The gene for IL-16 precursor has been localized to chromosome 15. The biological activities ascribed to IL-16 are reported to be dependent on the cell surface expression of CD4, suggesting that IL-16 is a CD4 ligand. Besides its chemotactic properties, IL-16 has also been shown to suppress HIV-1 replication in vitro. Recombinant E. coli-derived IL-16 produced at R&D Systems is present mostly as a monomer, exhibits chemotactic activity for lymphocytes at high concentrations, lacks chemotactic activites for monocytes, and binds the extracellular domain of CD4 with low affinity.

References
  1. Cruikshank, W.W. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5109.
  2. Baier, M. et al. (1997) Proc. Natl. Acad. Sci. USA 94:5273.
  3. Zhou, A. et al. (1997) Nature Medicine 3:659.
  4. Bazan, J.F. and T.J. Schall (1996) Nature 381:29.
Long Name
Interleukin 16
Entrez Gene IDs
3603 (Human); 16170 (Mouse)
Alternate Names
FLJ16806; FLJ42735; FLJ44234; HsT19289; IL16; IL-16; interleukin 16 (lymphocyte chemoattractant factor); LCF; LCFprIL-16; lymphocyte chemoattractant factor; neuronal interleukin 16; NIL16; PRIL16; prointerleukin 16; pro-interleukin-16

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